T淋巴细胞在甲型H1N1流感病毒感染中的变化及意义

目的 研究甲型H1N1流行性感冒（流感）患者外周血T淋巴细胞及其激活亚群的变化.方法 用流式细胞仪检测144例甲型HlNl流感患者和41例健康体检者淋巴细胞亚群,对其中83例患者进一步分析治疗前后T淋巴细胞及其激活亚群（HLA-DR＋CD3＋、HLA-DR＋CD4＋和HLA-DR＋CD8＋细胞）.结果 ①与对照组相比,H1N1并发肺炎组和H1N1组淋巴细胞数明显低于对照组,H1N1并发肺炎组淋巴细胞数明显低于H1N1组;H1N1并发肺炎组T淋巴细胞百分比明显低于对照组（P〈0.05）.②治疗前后CD3、CD8细胞百分比和绝对数均差异有统计学意义,治疗前显著降低,CD4细胞数治疗前显著降低.③治疗前后T淋巴细胞激活亚群（HLA-DR＋CD3＋、HLA-DR＋CD4＋和HLA-DR＋CD8＋细胞）百分比差异有统计学意义,治疗前显著降低.结论 测定甲型H1N1流感患者外周血T淋巴细胞及其激活亚群的变化有助于评价甲型H1N1流感患者感染早期的细胞免疫状况,可以作为甲型H1N1流感早期诊断的辅助指标.

Study on the changes of the T-lymphocyte subsets and their significance in influenza A(H1N1) virus patients

Objective To study the changes of T-Lymphocyte and activated T-Lymphocyte subsets in influenza A(H1N1)virus patients.Methods The percentages of the subsets of Lymphocyte were detected by flow cytometry in influenza A(H1N1) virus patients (n=144) and normal controls(n=41).Furthermore,the subsets of T-Lymphocyte and activated T-lymphocyte were analyzed in 83 among those patients before and after treatment.Results Compared with the control group,the counts of Lymphocyte in patients with influenza A(H1N1) virus was significantly discreased,the counts of Lymphocyte in patients with influenza A virus concurrent pneumonia was significantly discreased those of no concurrent pneumonia; Compared with the control group,the percentage of T-lymphocyte in patients with influenza A virus concurrent pneumonia was significantly discreased.The counts and percentage of CD3 and CD8 cells was significantly discreased in patients (n=83) before treatment;The counts of CD4 cells was significantly discreased before treatment.The percentage of HLA-DR+CD3+.HLA-DR+CD4+and HLA-DR+CD8+ cells was significantly discreased in patients (n=83) before treatment.Conclusions To understand the expression of the T-Lymphocyte and activated T-Lymphocyte subsets in influenza A (H1N1) virus patients may help to evaluate the patients'cellular immune status,but also be a guideline of early diagnosis of Influenza A(H1N1)virus.