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| HPLC-ELSD测定复方参芪软胶囊中黄芪甲苷的含量 | |
| 引用本文： | 王春艳,杨建春,于庆坤,张琦,潘卫三. HPLC-ELSD测定复方参芪软胶囊中黄芪甲苷的含量[J]. 中国现代应用药学, 2010, 27(9): 846-848 |
| 作者姓名： | 王春艳 杨建春 于庆坤 张琦 潘卫三 |
| 作者单位： | 1.唐山市妇幼保健院，河北 唐山 063000；2.沈阳药科大学药学院，沈阳 110016 |
| 摘 要： | 目的建立以高效液相色谱-蒸发光散射检测法测定复方参芪软胶囊中黄芪甲苷含量的方法。方法色谱柱为AgilentHypersil ODS,流动相为乙腈-水（35∶65）,流速为1.0 mL.min-1,进样量为20μL;蒸发光散射检测器条件：载气（压缩空气）压力为0.32 MPa,载气流量：2.6 L.min-1,漂移管温度为108℃。结果黄芪甲苷进样量的线性范围为0.402 4~4.024 0μg（r=0.999 8）;平均回收率为100.02%（RSD=1.41%）。结论该方法准确度高、专属性强,可用于该制剂中黄芪甲苷的含量测定。 |
| 关 键 词： | 高效液相色谱-蒸发光散射检测法 复方参芪软胶囊 黄芪甲苷 含量测定 |
Content Determination of Astragaloside in Compound Shenqi Soft Capsule by HPLC-ELSD |
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| WANG Chunyan,YANG Jianchun,YU Qingkun,ZHANG Qi,PAN Weisan. Content Determination of Astragaloside in Compound Shenqi Soft Capsule by HPLC-ELSD[J]. The Chinese Journal of Modern Applied Pharmacy, 2010, 27(9): 846-848 | |
| Authors: | WANG Chunyan YANG Jianchun YU Qingkun ZHANG Qi PAN Weisan |
| Affiliation: | 1.The Women and Children’s Hospital of Tangshan,Tangshan 063000,China;2.Shenyang Pharmaceutical University,Shenyang 110016,China) |
| Abstract: | OBJECTIVE To establish an HPLC-ELSD method for the determination of astragaloside in compound shenqi soft capsule.METHODS The samples were determined by HPLC-ELSD on an Agilent Hypersil ODS column using acetonitrile-water（35∶65） as the mobile phase at a flow rate of 1.0 mL.min-1.The injection volume was 20 μL.The ELSD conditions were as follows： pressure of compressed air was 0.32 MPa,the flow rate of compressed air was 2.6 L.min-1,drift temperature was 108 ℃.RESULTS The linear range of astragaloside was 0.402 4-4.024 0 μg（r=0.999 8） and the average recovery rate was 100.02%（RSD=1.41%）.CONCLUSION The method is accurate,specific and applicable for the determination of astragaloside in compound shenqi soft capsule. |
| Keywords: | HPLC-ELSD compound shenqi soft capsule astragaloside content determination |
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