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Comparison of dual-color dual-hapten brightfield in situ hybridization (DDISH) and fluorescence in situ hybridization in breast cancer HER2 assessment
Authors:Csaba Kosa  Laszlo Kardos  Judit Kovacs  Zoltan Szollosi
Affiliation:1. Department of Surgery, Medical and Health Science Center, University of Debrecen, Nagyerdei krt 98, H-4012 Debrecen, Hungary;2. Department of Infection Control Services, Kenezy County Hospital, Bartok Bela u 2-26, H-4043 Debrecen, Hungary;3. Department of Pathology, B-A-Z County Hospital, Szentpeteri kapu 72-76, H-3526 Miskolc, Hungary
Abstract:
The most optimal method for assessing HER2 status is still subject to controversy as far as the type of assay used, the optimal method to perform, and the costs of each assay are concerned. The current study was done as a validation study prior to setting up a clinical HER2 testing service using the new commercial dual-color dual-hapten brightfield in situ hybridization (DDISH), but it was felt that our experience may be of interest to other laboratories considering setting up HER2 diagnostic facilities.
Keywords:Breast cancer   HER2   Chromosome 17   FISH   DDISH
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