矿化蚕丝基骨材料的生物相容性及体内降解的观察

目的探讨仿生制备的矿化蚕丝基骨材料在大鼠体内的生物相容性及体内降解性。方法成年雄性SD大鼠16只,体重230-300克。在背部肌肉内和腹腔内植入矿化蚕丝基骨材料,观察时间阶段为2周,4周,6周,8周。结果分别在术后2周,4周,6周,8周,行大体观察,细胞毒性研究,组织学切片观察材料的情况,植入2周后出现炎症反应明显,材料框架基本存在,术后8周大鼠体内植入的矿化蚕丝基骨材料没有明显的排异反应,而且大部分被吸收。结论矿化蚕丝基骨材料具有良好的生物相容性和适宜的生物降解性能,是比较理想的骨缺损的替代材料。

Observation to biocompatibility and biological degradation of mineralized silk-fibroin nanocomposite in mice body

Objective To investigate biocompatibility and biological degradation of mineralized silk-fibroin nano composite in mice body. Methods Mineralized silk-fibroin nano composite were implanted into muscle of back and abdominal cavity of SD mature male mice that weight 230-300g. In the end of two week, four week, six week and eight week after surgery, the grafts were harvested and tested by on the whole observation, cytotoxicity research and histological examinations. Results The results showed that inflammatory reaction appeared obviously around mineralized silk-fibroin nano composites and nano composites were not absorbed after 2 weeks; but after 8 weeks , rejection did not appear, histological examination showed that most of nano composites were absorbed. Conclusion It is suggested that mineralized silk-fibroin nano composites that have good biocompatibility and biological degradation are very ideal substitute materials for bone defect.