金银花和山银花的薄层色谱指纹图谱分析

目的建立高效薄层指纹图谱定性鉴别方法,使用金银花对照提取物(JYH ERS)直接比较金银花和山银花药材的异同。方法使用高效薄层色谱法、硅胶G60薄层板,分别以乙酸丁酯-丙酮-甲酸-水(10∶1∶4∶4)和三氯甲烷-甲醇-水(12∶4∶1)为展开剂,用绿原酸、异绿原酸A、异绿原酸B、异绿原酸C、木犀草素-7-O-葡萄糖醛酸苷、断氧化马钱子苷和断马钱子酸对金银花及山银花进行定性鉴别。结果高效薄层色谱显示JYH ERS与金银花药材在整体图谱上具有一致性。与山银花药材比较,金银花及JYH ERS中的断马钱子酸斑点明显,两种不同的展开剂均能有效鉴别出金银花和山银花药材。结论金银花与山银花药材容易混淆,使用所建立的高效薄层指纹图谱鉴别方法及JYH ERS可快速、有效地区分金银花与山银花。

Fingerprint Analysis of Lonicerae japonicae Flos and Lonicerae Flos

Objective Our study assessed the application of JYH ERS in HPTLC identification of Lonicerae japonicae flos and Lonicerae flos.MethodsTLC was conducted on pre-coated silica gel 60 HPTLC plate,developing with the solvent system of butyl acetate-acetone-formic acid-water(10∶1∶4∶4)and trichloromethanemethanol-water(12∶4∶1)respectively. The contents of chlorogenic acid and isochlorogenic acid A were determined by external standard method using chlorogenic acid reference substance and isochlorogenic acid A reference substance,and the components in Lonicerae japonicae flos were identified by isochlorogenic acid B,isochlorogenic acid C,luteolin 7-O-glucuronide,secoxyloganin and secologanic acid.ResultsJYH ERS and Lonicerae japonicae flos are consistent in whole chromatograms. Compared with Lonicerae flos, the bands of secoxyloganin and secologanic acid were obvious in JYH ERS and Lonicerae japonicae flos, which could effectively distinguish Lonicerae japonicae flos and Lonicerae flos.ConclusionLonicerae japonicae flos and Lonicerae flos are easily confused and can be identified effectively by HPTLC. Using the current HPTLC method and JYH ERS can identify Lonicerae japonicae flos quickly and effectively.