硫酸锌联合阿司匹林治疗急性心肌梗死大鼠的效果及其作用机制

【目的】探讨硫酸锌联合阿司匹林治疗急性心肌梗死(AMI)大鼠的效果及其作用机制。【方法】选取健康成年SD雄性大鼠,随机分为假手术组(未建模,生理盐水灌胃)、模型组(建立AMI模型,生理盐水灌胃)、硫酸锌组建立AMI模型,硫酸锌75 mg/(kg·d)灌胃]、阿蚜匹林组建立AMI模型,阿司匹林75 mg/(kg·d)灌胃]、联合组建立AMI模型,硫酸锌与阿司匹林(2:1)120 mg/(kg·d)灌胃],连续处理7 d。检测并比较各组大鼠乳酸脱氢酶(LDH)、肌酸激酶(CK)、天门冬氨酸氨基转移酶(AST)、丙二醛(MDA)、超氧化物歧化酶(SOD)、谷胱甘肽过氧化物酶(GSH-Px)、左室内压最大上升速率(+dp/dtmax)、左室内压最大下降速率(-dp/dtmax)、心室开始收缩至发生dp/dtmax的间隔时间(t-dp/dtmax)、左室舒张末期压(LVEDP)、左室收缩压(LVSP)、心肌梗死面积比(MIS%)等值,观察各组心肌组织结构变化。【结果】模型组大鼠血清LDH、CK、AST水平显著高于假手术组,联合组大鼠血清LDH、CK、AST水平显著的低于模型组、硫酸锌组、阿司匹林组,其差异均有统计学意义(P<0.05)。模型组大鼠血清MDA水平显著高于假手术组,血清SOD及GSH-Px显著低于假手术组,其差异均有统计学意义(P<0.05);联合组大鼠血清MDA水平体于模型组、硫酸锌组、阿司匹林组,联合组大鼠血清SOD及GSH-Px高于模型组、硫酸锌组、阿司匹林组,其差异均有统计学意义(P<0.05)。模型组大鼠+dp/dtmax、-dp/dtmax、LVSP测定值显著低于假手术组(P<0.05),联合组的+dp/dtmax、-dp/dtmax、LVSP测定值显著高于模型组、硫酸锌组、阿司匹林组,其差异均有统计学意义(P

Effects of Zinc Sulfate Combined with Aspirin on Cardiac Function and Serum Enzymes in AMI Rats

【Objective】To investigate the effect and mechanism of zinc sulfate combined with aspirin in the treatment of acute myocardial infarction(AMI)rats.【Methods】Healthy adult SD male rats were randomly divided into the sham operation group(normal saline perfusion in no AMI model rats),the model group(equal amount of normal saline gavage in AMI model rats)9 the zinc sulfate groupzinc sulfate 75 mg/(kg·d)gavage in AMI model rats],the aspirin groupaspirin 75mg/(kg·d)gavage in AMI model rats],and the combined groupzinc sulfate and aspirin(2:1)with total dose of 120mg/(kg·d)gavage in AMI model rats].All rats kept continuous,treatment for 7d.Rat lactate dehydrogenase(LDH),creatine kinase(CK),aspartate aminotransferase(AST),malondialdehyde(MDA),superoxide dismutase(SOD),gluten Glutathione peroxidase(GSH-Px),the maximum rate of increase of left ventricular pressure(+dp/dtmax),the maximum rate of increase of left ventricular pressure(-dp/dtmax),the interval time from the beginning of ventricle contraction to the occurrence of dp/dtmax(T-dp/dtmax)9 left ventricular end diastolic pressure(LVEDP),left ventricular systolic pressure(LVSP),and myocardial infarction area ratio(MIS%)were detected and compared among groups.【Results】The serum LDH,CK,and AST levels of the model group were significantly higher than those of the sham operation group(P<0.05);the serum LDH,CK,and AST levels of the combination group were significantly lower than those of the model group,zinc sulfate group,and aspirin group(P<0.05).The serum MDA level of the modd group was significantly higher tlian that of the sham operation group(P<0.05),and the serum SOD and GSH-Px were significantly lower than that of the sham operation group(P<0.05).Serum MDA level in the combination group was lower than that in the model group,the zinc sulfate group and the aspirin group(P<0.05);while the serum levels of SOD and GSH-Px in the combination group were higher than those in the model group,the zinc sulfate group snd the aspirin group(P<0.05).The measured values of+dp/dtmax,-dp/dtmax and LVSP of the model group were significantly lower than those of the sham operation group(P<0.05),and the measured values of+dp/dtmax,dp/dtmax and LVSP of the combined group were significantly higher than those of the model group,the Zinc sulfate group and the aspirin group;the difference was statistically significant(P<0.05).The t-dp/dtmax,LVEDP and MIS% were significantly higher than the sham operation group.The values of t-dp/dtmax,LVEDP and MIS% in the combined group of rats were significantly lower than those in the model group,the zinc sulfate group,the aspirin group;and the difference was statistically significant(P<0.05).The myocardial tissues of rats in the sham operation group were arranged neatly,densely with clear structure.The cytoplasm was uniformly stained.The nucleus was located in the center of the cells.There was no iaflammatory cell infiltration in the myocardiunu The myocardial tissue of the AMI model group showed obvious structural damage,cell swelling,nuclear pyknosis and lysis,focal or flaky myocardial necrosis.The myocardial tissue ol the zinc sulfate group and the aspirin group still showed obvious necrotic areas,but it was less than the model group.The myocardial tissue of llie rata in the coiiibinatian group showed only punctate necrosis or vacuole areas,and the myocardial pathology was significantly reduced.【Conclusion】The results showed that zinc sulfate combined with aspirin treatment of AMI rats can significantly reduce myocardial infarct size,reduce myocardial enzymes and improve cardiac function in rats.