急性胰腺炎肠黏膜损伤情况及通透性改变的实验研究

目的：观察实验性重症急性胰腺炎（ANP）鼠肠通透性的改变,并观察肠黏膜形态变化及细菌移位的关系。方法：实验性大鼠41只,随机分为胰腺炎组和对照组。利用电子显微镜以硝酸镧作为踪剂观察肠黏膜通透性和肠细胞的渗透性。结果：回肠黏膜出现明显病理改变,肠黏膜通透性和肠细胞渗透性明显增高。胰腺炎组肠系膜淋巴结和腹水标本培养出肠道菌属者,结论：ANP时存在着严重的肠道屏障功能损伤。发病时肠黏膜即遭到严重破坏,肠通透性增高,从而引起肠道细菌移位。

An experimental study of intestinal permeability and injury in the intestinal mucosa in acute necrotizing pancreatitis

Objective:The changes of intestinal permeability and relationship of intestinal mucosa and bacterial translocation were studied in rat ANP models.Methods:41 Wistar rats were divided into two group,model group(n=21) and control group(n=20).The intestinal permeability was observed by lanthanum tracer under eletron microscope.Results:The pathologic lesions were found in the intestinal villus of the ANP group,including acute necrosis of intestinal mucosa and increased permeability of intestinal mucosa and enterocytes.Bacterial translocation to organs and ascites could be found in all animals of ANP group.Conclusions:The results demonstrated that there was a severe gut barrier damage and injury in the intestinal mucosa,which led to bacterial translocation from intestine as the source of pancreatic infection.