| HPLC法测定不同产地千金子中秦皮乙素的含量 |
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| 引用本文： | 李英霞,胡乃合,苏本正,梁焕,杜杰. HPLC法测定不同产地千金子中秦皮乙素的含量[J]. 辽宁中医杂志, 2009, 36(6): 996-997 |
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| 作者姓名： | 李英霞 胡乃合 苏本正 梁焕 杜杰 |
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| 作者单位： | 山东省中医药研究院,山东,济南,250014;山东中医药大学,山东,济南,250014;中国药材集团公司,北京,100055 |
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| 基金项目： | “十一五”国家支撑计划基金资助项目(2006BAI09B06-05) |
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| 摘 要： | 目的:对不同地区千金子药材中秦皮乙素的含量进行测定。方法:采用高效液相色谱法,以乙腈-水-冰醋酸(12∶88∶0.08)为流动相,使用C18柱,检测波长340nm。结果:秦皮乙素在0.05216~0.3651μg范围内,线性关系良好,r=0.9998,平均回收率为98.98%,各地千金子药材中秦皮乙素含量一般在0.0020%~0.0098%。结论:采用高效液相色谱法测定千金子中秦皮乙素的含量,方法准确,重复性好,为控制千金子药材的质量提供了科学方法。
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| 关 键 词： | 千金子 秦皮乙素 高效液相色谱 含量测定 |
The contents of aesculetion in semen Euphorbiae from different habitats were determined by HPLC |
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| LI Ying-xia,Hu Nan-he,SU Ben-zheng,LIANG Huan,DU Jie. The contents of aesculetion in semen Euphorbiae from different habitats were determined by HPLC[J]. Liaoning Journal of Traditional Chinese Medicine, 2009, 36(6): 996-997 |
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| Authors: | LI Ying-xia Hu Nan-he SU Ben-zheng LIANG Huan DU Jie |
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| Affiliation: | 1.Shandong Academy of Chinese Medicine;Jinan 250014;Shandong;China;2.Shandong University of Chinese Medicine;3.Chinense Medicinal Materials Company Group;Beijing 100055;China |
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| Abstract: | Objective:To determine the contents of the Semen Euphorbiae from different habitats.Methods:The contents of aesculetion in semen Euphorbiae from six different habitats were determined by HPLC.The column of kromasil C18 was used.The mobile phase was acetonitrile-water-acetic acid(12∶88∶0.08)and detection wavelength was 340nm.Results:The results showed that aesculetin had a good Linear relationship in the concentrations between 0.05216μg to 0.3651μg(r=0.9998).The contents of aesculetin from different habitats material were most between 0.002% to 0.0098%.Conclusion:This accuracy of this method of determining the content of aesculetin in Semen Euphorbiae by HPLC is high and the reproduction quality is good.The scientific basis is provided to be used to control the quality of Semen Euphorbiae. |
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| Keywords: | Semen Euphorbiae aesculetin HPLC determination of content |
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