结肠癌患者肿瘤组织及外周血ICAM-1和VCAM-1表达的研究

目的:检测结肠癌患者肿瘤组织中细胞间粘附分子-1(intercellular adhension molecular-1,I-CAM-1)和血管细胞粘附分子-1(vascular cell adhesion molecular-1,VCAM-1)的表达,同时测定血清中可溶性ICAM-1(sICAM-l)和可溶性VCAM-1(sVCAM-1)水平,探讨二者与结肠癌生物学特性及预后的关系。方法:应用免疫组化SP法对32例结肠癌组织、17例溃疡性结肠炎组织和21例正常结肠组织标本进行ICAM-1与VCAM-1表达检测,分析其与结肠癌临床病理指标的关系。ELISA法检测结肠癌患者手术前后外周血sICAM-1和sVCAM-1含量,分析治疗前后水平的变化。结果:结肠癌组织ICAM-1和VCAM-1的阳性表达率显著高于溃疡性结肠炎组织(P<0.05)及正常结肠组织(P<0.01),其表达水平与患者年龄、性别、肿瘤大小和病理学分级无关(P>0.05),与肿瘤分期及有无远处转移有关(P<0.05);结肠癌患者外周血sICAM-1和sVCAM-1水平明显高于溃疡性结肠炎患者(P<0.05)及正常对照组(P<0.01),结肠癌患者术后1周sICAM-1及sVCAM-1水平明显下降(P<0.01,P<0.01)。结论:结肠癌患者肿瘤组织及外周血ICAM-1和VCAM-1的表达可能与结肠癌的浸润转移有关;sICAM-1、sVCAM-1的检测可能成为监测结肠癌病情进展和评价治疗效果的有价值指标。

Study of intercellular adhesion molecule-1 and vascular cell adhesion molecule-1 expressions in cancer tissue and serum of patients with colon cancer

Objectives:To detect the expressions of ICAM-1,VCAM-1 in colon cancer tissues and to measure the serum soluble intercellular adhesion molecular-l(sICAM-l)and soluble vascular cell adhesion molecular-1(sVCAM-l).Methods:Specimens from 32 colon cancer patients,17 ulcerative colitis patients,21 normal tissues were observed by immunohistochemistry.ELSIA technique was used to measure serum concentration of sICAM-1 and sVCAM-1 before and post operation in colon cancer patients.Results:The expressions of ICAM-1 and VCAM-l in colon cancer tissues were significantly higher than those in ulcerative colitis tissues(P<0.05)and in normal control group(P<0.01).Their expressions were not related to age,sex,tumor size and pathological grade(P>0.05),but had significantly statistic difference between TNM stage and metastasis(P<0.01).The level of sICAM-1 and sVCAM-1 in serum of colon cancer patients were significantly higher than those in ulcerative colitis patients(P<0.05)and in health control group(P<0.01).The levels in colon cancer 7 days postoperatively decreased significantly(P<0.01).Conclusions:The expressions of ICAM-1and VCAM-1 may be related with invasion,metastasis of colon cancer.The measurement of serum sICAM-1 and sVCAM-1 may be valuable markers to evaluate the progression,therapeutic evaluation for patients with colon cancer.