上海市市售水产品中副溶血性弧菌的分离、鉴定及耐药性研究

目的了解上海市市售水产品中副溶血性弧菌(Vibrio parahaemolyticus,Vp)污染状况和耐药性,为防治Vp引起的食源性疾病提供依据。方法采集上海市各大农贸市场3类水产品共273份检测Vp,用统计学处理分析结果;K-B法进行药敏试验。结果水产品中Vp平均检出率为38.46%,其中甲壳类的检出率为50.96%,贝类为27.12%,鱼类为15.79%,三者间有极显著差异(P<0.01)。药敏试验结果显示,105株分离菌株对头孢曲松、萘啶酸和诺氟沙星100%敏感,对氨苄西林的耐药率达69.52%,有20株对多种抗生素耐药。结论上海市售水产品中Vp污染比较严重,药敏结果对多种抗生素耐药。

Isolation, identification and drug-resistance of Vibrio parahaemolyticus isolated from the aquatic products in Shanghai markets

The aim of this study is to understand the condition of contamination and drug resistance of Vibrio parahae-molyticus （Vp） isolated in the aquatic products in the retail market of Shanghai, and provide evidence for prevention and treatment of disease caused by this organism. From May to July of 2008, 273 aquatic products of three species from the fish markets in Shanghai were collected to isolate Vp. It was found that the average rates of contamination with Vp was 38.46 %, the rates of contamination in crustacean, shellfish and fishes were 50.96%, 27. 12%, and 15. 79% respectively, with a significant difference in the prevalence（P〈0.01）. All isolates tested for susceptibility to 10 antibiotics by K-B method. Of the 105 isolates examined, all strains were sensitive to ceftriaxone, nalidixic acid and norfloxacin. The resistant rates to ampicillin was 69. 52%. while 20 Vp strains showed multiple resistance to many antibiotics. It is concluded that the aquatic products retailed in Shanghai were seriously contaminated by Vp, showing a multi-drug resistance pattern.