| 新生隐球菌荚膜基因CAP60对菌体荚膜形成的影响 |
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| 引用本文： | 潘炜华,廖万清,顾菊林,霍克克. 新生隐球菌荚膜基因CAP60对菌体荚膜形成的影响[J]. 第二军医大学学报, 2002, 23(12): 1339-1341 |
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| 作者姓名： | 潘炜华 廖万清 顾菊林 霍克克 |
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| 作者单位： | 第二军医大学长征医院皮肤性病科,上海,200003;复旦大学遗传学研究所 |
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| 基金项目： | 国家自然科学基金资助项目 (3 95 70 0 43 ) |
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| 摘 要： | 目的：研究新生隐球菌荚膜相关基因CAP60对菌体荚膜形成的影响。方法：用PCR方法分离CAP60基因并测序鉴定,将该基因导入转化载体并转化cap60ura^-菌株,乳胶凝集试剂盒筛选转化分子。结果：部分转化子恢复莱膜的表型,表明得到的片段具有荚膜基因功能。结论：荚膜基因对新生隐球菌的荚膜形成都是必须的,缺失其中的任何一个基因都会使新生隐球菌变成无荚膜表型。
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| 关 键 词： | 新生隐球菌 CAP60 荚膜 |
| 文章编号： | 0258-879X(2002)12-1339-03 |
| 修稿时间： | 2002-04-01 |
Influence of capsule-associated gene CAP60 on capsule forming of Cryptococcus neoformans |
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| PAN Wei Hua ,LIAO Wan Qing ,GU Ju Lin ,HUO Ke Ke. Influence of capsule-associated gene CAP60 on capsule forming of Cryptococcus neoformans[J]. Former Academic Journal of Second Military Medical University, 2002, 23(12): 1339-1341 |
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| Authors: | PAN Wei Hua LIAO Wan Qing GU Ju Lin HUO Ke Ke |
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| Affiliation: | PAN Wei Hua 1,LIAO Wan Qing 1,GU Ju Lin 1,HUO Ke Ke 2 |
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| Abstract: | Objective: To study the influence of capsule associated gene CAP60 on the capsule forming of Cryptococcus neoformans .Methods: The capsular associated gene CAP60 was obtained by PCR and sequenced for verification.The fragment was inserted into vector and then transfected into the reform plasmid into ura -,capsule deficient strain cap60.Results:Some of the transformants regained the capsule phenotype,indicating the fragment had capsular gene function.Conclusion:Capsular gene of C.neoformans is necessary for its capsule forming,the deficiency of any capsular gene may lead to a capsule deficient phenotype. |
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| Keywords: | Cryptococcus neoformans CAP60 capsule |
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