黄连解毒汤对2型糖尿病大鼠血糖和血脂代谢的影响

背景黄连解毒汤是清热解毒代表方,治疗多种非感染性疾病有较好疗效,但实验研究证据尚较缺乏.目的研究黄连解毒汤对实验性2型糖尿病大鼠的血糖、血脂代谢的影响.设计随机对照的实验研究.地点、材料和干预实验在华中科技大学同济医学院实验动物中心清洁级动物实验室进行.实验选用清洁级雄性Wistar大鼠42只,用脲佐菌素配合高脂饲料饲养方法建立2型糖尿病模型,分为黄连解毒汤组、二甲双胍组、模型组各11只,采用相应药物治疗8周,另设9只作为正常对照组.主要观察指标①治疗4周后每组大鼠口服葡萄糖耐量检测结果.②治疗8周后每组大鼠血清总胆固醇、三酰甘油、高密度脂蛋白胆固醇(highdensitylipoprotein cholesterol,HDL-C)、载脂蛋白A1(apolipoprotein A1,ApoA1)、载脂蛋白B(apolipoprotein B,ApoB)、空腹血糖、空腹血清胰岛素、平均日进食量、体质量检测结果.结果黄连解毒汤组总胆固醇(1.91±0.23)mmol/L]、三酰甘油(0.41±0.72)mmol/L]、ApoB(0.37±0.03)mmol/L]、空腹血糖(7.1±0.9)mmol/L]、进食量(19.0±0.7)g]、大鼠体质量(308.7±9.9)g]低于模型组(3.18±0.17),(1.85±0.73),(0.46±0.04),(8.9±1.3)mmol/L,(21.3±1.9),(342.5±20.1)g](F=9.6,P＜0.05;F=15.2～41.2,P＜0.01);同时改善了糖耐量.结论黄连解毒汤具有降糖和调脂作用.

Effects of huanglian jedu decoction on blood glucose and lipid metabolism in type 2 diabetic rats

BACKGROUND:Huanglian Jiedu decoction (HLJDD) is effective in treating none-infectious diseases.However, there has not been any evidences from experimental investigation.OBJECTIVE:To study the effects of HLJDD on the blood glucose and lipid metabolism of experimental type 2 diabetes mellitus rats.DESIGN:Randomized and controlled experimental study.SETTING, PARTICIPANTS and INTERVENTION:Experiments were done in standard rat feeding room in Laboratory Animal Center, Tongji Medical College, Huazhong University of Science & Technology.Male Wistar rats were used to be induced into type 2 diabetes by streptozotocin and high fat laboratory feed, HLJDD, mefformin, were orally administered to the rats for 8 weeks.Model group and normal group were set up at the same time.MAIN OUTCOME MEASURES:Results of oral glucose tolerance test (OGTT) was determined after treatment for 4 weeks, and total cholesterol,triglycerides, high density lipoprotein(HDL), apolipoprotein A Ⅰ (APOA I),apolipoprotein B (ApoB), fasting blood glucose ( FBG ), fasting serum insulin (INS), eating mass and body mass were determined.RESULTS:HLJDD Decoction decreased the level of total cholesterol (1.91 ±0.23 vs 2.18±0.17,F=9.6, P ＜0.05), triglycerides (0.41 ±0.72 vs 1.85±0.73, F=22.3, P ＜ 0.01), APOB(0.37±0.03 vs 0.37±0.01, F=17.5, P ＜0.01) and FBG (7.1±0.9 vs8.9±1.3,F=41.2, P ＜0.01); raised the level of HDL(1.37 ± 0.16 vs 1.07±0.13, F=14.0, P ＜0.01), and APOA Ⅰ (0.78±0.04 vs 0.71 ±0.06,F = 125.7, P ＜ 0.05); ameliorated glucose tolerance; and reduced the eating mass(19.0±0.7 vs 21.3±1.9, F=18.5, P ＜0.01) andbody mass (308.7±9.9 vs 342.5±20.1, F= 15.2, P ＜0.01) of the rats.CONCLUSION:HLJDD Decoction not only has the hypoglycemic property,but also modulate the blood lipids.