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| 硫醇自组膜上葡萄糖氧化酶多层有序膜的组装及应用 | |
| 引用本文： | 陈仲道 朱军. 硫醇自组膜上葡萄糖氧化酶多层有序膜的组装及应用[J]. 济宁医学院学报, 1997, 20(3): 13-14 |
| 作者姓名： | 陈仲道 朱军 |
| 作者单位： | 济宁医学院化学教研室 |
| 摘 要： | 本文首先在金电极表面形成表面３－巯基１－丙磺酸自组装膜。研究了自组装膜表面葡萄糖氧化酶和二茂铁取代聚（４－乙烯基吡啶）多层有序膜的形成及其电化学特性。电极对葡萄糖测定的线性范围为０．１￣１０ｍｍｏｌ／Ｌ，检测限为０．０１ｍｍｏｌ／Ｌ，抗坏血酸对测定不产生影响。 |
| 关 键 词： | 葡萄糖氧化酶 自组装膜 多层有序膜 |
A GLUCOSE AMPEROMETRIC ENZYME ELECTRODE BY IMMOBILIZED GLUCOSE OXIDASE IN A FERROCENE ATTACHED POLYYINYLPYRIDINE MULTILAYER FILM |
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| Chen Zhongdao,Zhu Jun,Hou Shifeng,et al.. A GLUCOSE AMPEROMETRIC ENZYME ELECTRODE BY IMMOBILIZED GLUCOSE OXIDASE IN A FERROCENE ATTACHED POLYYINYLPYRIDINE MULTILAYER FILM[J]. Jounal of Jinning Medical College, 1997, 20(3): 13-14 | |
| Authors: | Chen Zhongdao Zhu Jun Hou Shifeng et al. |
| Abstract: | An amperometric enzyme electrode for glucose has been prepared by immobilized glucose oxidase (GOD) in a multilayer film. The multilayer film of GOD and ferrocene attached poly(4-vinylpyridine) (Fc-PVP) complex is formed by elecrtrostatic force. The linear range of the enzyme elecrtode is extxnded up to 10mmol/L of glucose due to the addition of 5 GOD layers, and the detection limit is down to 0 01mmol/L. Ascorbic acid and uric acid have no imterference in the determination of glucose. |
| Keywords: | multilayer film glucose oxidase oxidase ferrocenr-attached ploy(4-vinylpyridine) |
| 本文献已被 CNKI 维普 等数据库收录！ | |