甲状旁腺激素对人牙乳头间充质细胞矿化能力的影响

目的：观察体外培养的人牙乳头间充质细胞（ｄｅｎｔａｌ ｐａｐｉｌｌａ ｍｅｓｅｎｃｈｙｍａｌ ｃｅｌｌ，ＤＰＭＣ）矿化特征及甲状旁腺激素（ｐａｒａｔｈｙｒｏｉｄ ｈｏｒｍｏｎｅ，ＰＴＨ）对人ＤＰＭＣ矿化特征的影响，以深入研究成牙本质细胞的分化和牙本质形成。方法：第４代人ＤＰＭＣ在含３３．３ｎｍｏｌ／Ｌ ＰＴＨ的矿化液中连续培养３５ｄ后，进行组织学染色、透射电镜（ＴＥＭ）和扫描电镜（ＳＥＭ）观察。用

The effects of parathyroid hormone on the calcification ability of cultured human dental papilla mesenchymal cell

AIM: Observation of the effects of parathyroid hormone on the calcification ability of cultured human dental papilla mesenchymal cell (DPMC). METHODS: Two groups of the fourth passage human DPMC were cultured for 35days and observed by transmission electron microscope (TEM), scanning electron microscope (SEM) and hght microscope (LM). The control group was cultured in DMEM medium supplemented with 15% FCS, 50mg/L ascorbic acid and 2mmol/L 3 - glycerophosphate. The PTH group was cultured in the medium of control group containing 33.3umol/L PTH. RESULTS:The cell presented mineralized nodules as well as matrix vesicles and had the same ultrastructure with odontoblasts under TEM. The addition of PTH in the medium caused not only a significant increase in the number of nodules but also the formation of many matrix vesicles. CONCLUSION: The results suggest that cultured human DMPC have the calcification ability which may be related to the matrix vesicles and PTH enhances the calcification ability of cultured human DPMC.