| HPLC法测定黄菊消银丸中绿原酸的含量 |
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| 引用本文： | 朱德杰. HPLC法测定黄菊消银丸中绿原酸的含量[J]. 中国中医药现代远程教育, 2013, 11(11): 151-152 |
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| 作者姓名： | 朱德杰 |
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| 作者单位： | 河南省周口市食品药品检验所,周口,466000 |
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| 摘 要： | 目的建立黄菊消银丸中绿原酸的含量测定方法。方法采用HPLC法,色谱条件为：色谱柱：EclipseXDB-C18;流动相：乙腈-0.4%磷酸溶液（13：87）;流速：1.0ml～min-1;检测波长：327nm。结果绿原酸在0.2556～0.7668mg范围内线性关系良好（r=0.9999）,平均回收率为98.87%,RSD=1.16%（n=6）。结论本方法操作简便,专属性强,结果准确,可用于黄菊消银丸的质量控制。
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| 关 键 词： | 绿原酸 高效液相色谱法 黄菊消银丸 |
Determination of Chlorogenic Acid in Huangjuxiaoyin Pill by HPLC |
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| Zhu Dejie. Determination of Chlorogenic Acid in Huangjuxiaoyin Pill by HPLC[J]. Chinese Medicine Modern Distance Education of China, 2013, 11(11): 151-152 |
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| Authors: | Zhu Dejie |
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| Abstract: | Objective To set up amethod to determination of chlorogenic acid in huangjuxiaoyin pill. Methods The content of chlorogenic acid determined by HPLC. The chromatographic system consisted of G18 colunm,using acetonitrile-0. 4% phasphare mlution（17 ： 83）asmobilc phasc. Thc content was detcctcd at a wavclcng of 327 nm,thc flow ratc was 1.0ml·min-1 . Results The chlorogenic acid have agood tincar rolatim in the range of 0. 2556-0. 7668mg（ r = 0. 9999）, the average recovery was 98.87 % and RSD = 1.16 % （ n = 6）. Conclusion The establishedmethod is simple,sensitive and accuracy . It can be used for quality control of huangjuxiaoyin pill. |
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| Keywords: | Chlorogenic acid HPLC Huangjuxiaoyin Pill |
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