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| 杜氏盐藻硝酸盐还原酶缺陷型突变藻株的分离和初步鉴定 | |
| 引用本文： | 贾岩龙,侯卫红,李杰,刘红涛,陈华燕,王建民,薛乐勋. 杜氏盐藻硝酸盐还原酶缺陷型突变藻株的分离和初步鉴定[J]. 郑州大学学报(医学版), 2005, 40(2): 245-247 |
| 作者姓名： | 贾岩龙 侯卫红 李杰 刘红涛 陈华燕 王建民 薛乐勋 |
| 作者单位： | 郑州大学细胞生物学研究室,郑州,450052 |
| 基金项目： | 国家高技术研究发展(863)计划 2002AA628050，国家自然科学基金资助项目 30270031 |
| 摘 要： | 目的：分离制备杜氏盐藻硝酸盐还原酶(NR)缺陷型突变藻株并进行初步鉴定。方法：①杜氏盐藻经乙基亚硝基脲(ENU)诱变后，利用氯酸盐抗性筛选方法获得氯酸盐抗性藻株，经复筛和不同氮源生长实验，获得NR缺陷型突变藻株。②用磺胺比色法测定酶反应生成的亚硝酸盐(NO2^-)的浓度，从而计算出盐藻NR活性。比较突变藻株与野生型杜氏盐藻NR活性，作出初步鉴定。结果与结论：共获得30株NR缺陷型突变藻株，共有6株NR活性显著降低。杜氏盐藻NR缺陷型突变藻株成功建立。 |
| 关 键 词： | 杜氏盐藻 硝酸盐还原酶 突变株 |
| 修稿时间： | 2004-08-24 |
Isolation and identification of nitrate reductase-deficient mutants of Dunaliella salina |
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| JIA Yanlong,HOU Weihong,LI Jie,LIU Hongtao,CHEN Huayan,WANG Jianmin,XUE Lexun. Isolation and identification of nitrate reductase-deficient mutants of Dunaliella salina[J]. Journal of Zhengzhou University: Med Sci, 2005, 40(2): 245-247 | |
| Authors: | JIA Yanlong HOU Weihong LI Jie LIU Hongtao CHEN Huayan WANG Jianmin XUE Lexun |
| Affiliation: | JIA Yanlong,HOU Weihong,LI Jie,LIU Hongtao,CHEN Huayan,WANG Jianmin,XUE Lexun Laboratory for Cell Biology,Zhengzhou University,Zhengzhou 450052 |
| Abstract: | Aim:To isolate and identify nitrate reductase(NR)-deficient mutants of Dunaliella salina (D.salina).Methods: After ethynitrosourea mutagenization, chlorate-resistant mutants were acquired by chlorate-resistant selection. By reselection and growing experiments with different nitrogen cultures, NR-deficient mutants were got. The concentration of nitrite produced in enzyme reaction was determined by sulfanilamide colormetry and then NR activity was calculated. Comparing NR activity of NR-deficient mutants with wild type D. salina, the mutants were identified. Results and Conclusion:Thirty NR-deficient mutants were obtained,and NR activity of 6 mutants decreased significantly. NR-deficient mutants of D.salina were established successfully. |
| Keywords: | Dunaliella salina nitrate reductase mutant |
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