虾青素对大鼠肝癌CBRH-7919细胞骨架和nm23蛋白的影响

目的研究虾青素(Astaxanthin,Asta)对大鼠肝癌细胞CBRH-7919细胞骨架和肿瘤转移抑制蛋白23(non-metastasis23,nm23)的影响。方法 MTT法检测Asta作用下对CBRH-7919细胞生长的影响,激光扫描共聚焦显微镜观测CBRH-7919细胞骨架的变化,免疫荧光法检测nm23蛋白的表达和分布。结果 Asta能够抑制CBRH-7919细胞的生长,激光扫描共聚焦显微镜观察到Asta对CBRH-7919细胞骨架有破坏作用,骨架网状结构降解、凝聚、分布不均,Asta作用时间越长对细胞骨架的破坏作用越明显。免疫荧光结果显示nm23蛋白主要分布在细胞质中,Asta作用下nm23蛋白在0～18 h内表达上调,18 h后表达下调。结论 Asta能显著抑制CBRH-7919细胞生长,可能与其破坏肿瘤细胞骨架及对nm23蛋白的调控有关。

Effect of Astaxanthin on cytoskeleton and nm23 protein in rat hepatocellular carcinoma CBRH-7919 cells

Objective To study the effect of astaxanthin on cytoskeleton and non-metastasis 23(nm23) protein in rat hepatocellular carcinoma CBRH-7919 cells.Methods Screening of sensitive cancer cell lines with astaxanthin using methyl thiazolyl tetrazolium colorimetric assay.Changes of cytoskeleton were observed by laser scanning confocal microscope.Expression of non-metastasis 23 protein was detected by immunofluorescence.Results Astaxanthin inhibited the proliferation of three tumor cell lines in a dose-and time-dependent manner.The inhibition was most pronounced in CBRH-7919 cell line with the half maximal inhibitory concentration(IC50) at 39 uM.This dose of astaxanthin and CBRH-7919 cell line were chosen for further studies.The cytoskeleton network was degradated,cohesive and unevenly distributed.Expression of nm23 protein was up-regulated in 0-18 h,but down-regulated after 18h.Conclusion Astaxanthin showed promising cytotoxic activity in CBRH-7919 cells.This is possible related with its impact on the cytoskeleton and regulation of nm23.