胶质纤维酸性蛋白免疫组织化学检测判断儿童畸胎瘤内神经组织的成熟程度

目的 应用胶质纤维酸性蛋白(GFAP)免疫组织化学染色检测儿童畸胎瘤内神经组织的分化程度,并分析其在区分成熟和未成熟畸胎瘤中的作用.方法 对2001年1月～2006年12月两院保存已经病理确定的儿童畸胎瘤标本(未成熟畸胎瘤10例,成熟畸胎瘤45例中的神经组织进行提取后按分化成熟和未成熟的神经组织分组,分别进行GFAP免疫组织化学染色,对结果作半定量分析,并作统计学处理.结果 显示未成熟畸胎瘤中GFAP检测为低表达,而成熟畸胎瘤为高表达,二组比较差异有统计学意义(P=0.0001).结论 可将GFAP列入检测畸胎瘤成熟度的参考指标.

Immunohistochemical assay of GFAP to determine the maturity of nerve tissue of teratoma in children

Objective To analyze the role of irnmunohistochemichal assay of glial fibrillary acidic protein (GFAP) in determining the maturity of nerve tissue of teratoma in children.Methods Sixty specimens of teratoma which were embedded by paraffin during January 2000 and December 2006 in Xinhua Hospital and Shanghai Children's Medical Center were chosen under the guidance of pathologists.All the specimens contained nerve tissue and divided into mature or immature group according to pathological diagnosis.In all the 60 eases,3(1 were boys and 30 were girls,aging from 2 days to 12.5 years.Ten cases were included in the immature group,in which 4 cases were in grade 1,3 in grade 2 and 3 in grade 3; 45 cases were included in the mature group.(Other 5 specimens were included in neither of the 2 groups because the sections escaped from tissue mieroarrays or the staining was diffieuh to observe.) Immunohistoehemieal staining of tissue mieroarrays was made to analyze the GFAP semi-quantitatively.The results were analyzed by SAS 6.10.Results Immature teratomas had low expression of GFAP,while mature teratomas had significantly higher expression than that in the immature group (p=0.0001).The expression of GFAP of neuroblastornas in the control group was negative.Conclusions The expression of GFAP can be an indicator to determine the maturity of teratoma,which can improve the efficiency of pathological diagnosis and provide immunohistochemical basis for classification of teratomas.