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| 巨噬细胞集落刺激因子对喉鳞状细胞癌喉癌细胞株的作用 | |
| 引用本文： | 葛平江,张宝泉,张建民,高志强,孔庆利,何维. 巨噬细胞集落刺激因子对喉鳞状细胞癌喉癌细胞株的作用[J]. 临床耳鼻咽喉头颈外科杂志, 2005, 19(6): 265-268 |
| 作者姓名： | 葛平江 张宝泉 张建民 高志强 孔庆利 何维 |
| 作者单位： | 广东省人民医院耳鼻咽喉-头颈外科,广州,510080;中国医学科学院,北京协和医学院,北京协和医院耳鼻咽喉科;中国医学科学院,中国北京协和医学院,基础研究所免疫室 |
| 基金项目： | 国家973重点课题基金资助项目(No2001CB5100009) |
| 摘 要： | 目的:了解和探讨巨噬细胞集落刺激因子(MCSF)及其受体对喉鳞状细胞癌细胞株(Hep2)细胞系的作用。方法:使用氚标记胸腺嘧啶核苷(3HTDR)掺入法和MTT法测试MCSF作用于Hep2后细胞的增殖状况,并测试加入巨噬细胞集落刺激因子受体(MC-SFR)抗体后细胞生长情况。ELISA法测试M-CSF作用Hep2细胞后上清中的MCSF的浓度变化。结果:Hep2细胞培养后较培养前的上清中的MCSF的浓度升高。加入的M-CSF浓度越大,细胞增殖越差,加入MCSFR抗体浓度越大细胞增殖越好。结论:MCSF对喉癌细胞Hep2细胞的增殖具有抑制作用。 |
| 关 键 词： | 喉肿瘤 癌 鳞状细胞 巨噬细胞集落刺激因子 增殖 Hep-2细胞系 |
| 文章编号： | 1001-1781(2005)06-0265-04 |
| 修稿时间： | 2004-10-09 |
The effect of macrophage colony stimulating factor on laryngeal cancer Hep-2 cell line |
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| GE Pingjiang,Zhang Baoquan,ZHANG Jianmin,GAO Zhiqiang,KONG Qingli,HE Wei. The effect of macrophage colony stimulating factor on laryngeal cancer Hep-2 cell line[J]. Journal of clinical otorhinolaryngology, head, and neck surgery, 2005, 19(6): 265-268 | |
| Authors: | GE Pingjiang Zhang Baoquan ZHANG Jianmin GAO Zhiqiang KONG Qingli HE Wei |
| Affiliation: | Department of Otolaryngology-Head and Neck Surgery, Guangdong Province People's Hospital, Guangzhou, 510080, China. |
| Abstract: | Objective:To investigate the possible role of M-CSF and its receptor in the laryngeal carcinoma Hep-2 cell line.Method:Cell proliferation was assayed by MTT analysis and thymidine incorporationx,after Hep-2 cell line culturing with M-CSF and M-CSFR.M-CSF concentration of surpernatant measured by a ELISA technique. Immunohistochemistry was performed on Hep-2 cell line and laryngeal carcinoma tissue.Result:The concentration of M-CSF increased after cell culturing.M-CSF can reduced the Hep-2 cell count.The action can be blocked by Anti-M-CSFR.Conclusion:Our observations support the hypothesis that M-CSF may inhibit the growth and differentiation in Hep-2 cell line. |
| Keywords: | Laryngeal neoplasms Carcinoma squamous cell Macrophage colony stimulating factor Proliferation Hep-2 |
| 本文献已被 CNKI 维普 万方数据 等数据库收录！ | |