| 体外感染实验探讨丙型肝炎病毒的ADE分子机制 |
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| 引用本文： | 聂青和,罗新栋,黄晓峰,张亚飞,程勇前.体外感染实验探讨丙型肝炎病毒的ADE分子机制[J].实用肝脏病杂志,2010,13(1):4-8. |
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| 作者姓名： | 聂青和 罗新栋 黄晓峰 张亚飞 程勇前 |
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| 作者单位： | 1. 第四军医大学唐都医院感染病科/全军感染病诊疗中心,西安市,710038
2. 电子显微镜室 |
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| 基金项目： | 国家自然科学基金资助项目(No:30771891) |
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| 摘 要： | 目的研究丙型肝炎病毒(HCV)在感染人滋养层细胞的过程中是否存在抗体依赖性感染增强(ADE)作用,以探讨HCV母婴传播的分子机制。方法将HCV阳性血清以4种不同方式感染人滋养层细胞,以免疫电镜观察HCV病毒颗粒在滋养层细胞中的表达,应用RT-PCR法、免疫组化法检测细胞内外的HCVRNA正链、负链,HCVNS5、NS3及C区抗原。结果在滋养层细胞胞浆内发现了HCV病毒颗粒,HCVRNA正链、负链,HCVNS5、NS3、C区抗原仅在全血清感染细胞内或上清中间断测得。结论HCV可以在滋养层细胞中复制。HCV感染滋养层细胞的过程中存在ADE机制,抗体和补体均参与了HCV的跨膜转运过程。
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| 关 键 词： | 丙型肝炎病毒 人滋养层细胞 体外感染 |
Experimental study of in vitro infection of human trophoblastic cells with hepatitis C virus |
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| NIE Qinghe,LUO Xindong,HUANG Xiaofeng,et al..Experimental study of in vitro infection of human trophoblastic cells with hepatitis C virus[J].Journal of Clinical Hepatology,2010,13(1):4-8. |
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| Authors: | NIE Qinghe LUO Xindong HUANG Xiaofeng |
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| Institution: | NIE Qinghe,LUO Xindong,HUANG Xiaofeng,et al. The Center of Diagnosis , Treatment for Infectious Diseases of PLA,Tangdu Hospital,The Fourth Military Medical University,Xi'an 710038 |
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| Abstract: | Objective To assess the antibody-dependent enhancement of infection(ADE) when the cultured trophoblastic cells were infected by hepatitis C virus (HCV). Methods We incubated the cytotrophoblasts with HCV by four different methods. Under the immunoelectron microscopy,we observed the HCV viral particles. RT-PCR method and immunohistochemistry were employed to assay the expressions of HCV NS5 and NS3 and HCV-C antigen and HCV RNA in cells or supernatant. Results The HCV viral particles were observed in the cul... |
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| Keywords: | Hepatitis C virus Cytotrophoblasts In vitro |
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