长链非编码RNA TPT1-AS1促进肝癌细胞增殖、迁移、侵袭的作用研究

目的 研究肿瘤蛋白翻译调节因子1反义-RNA1(TPT1-AS1)在肝细胞和肝癌细胞中表达的差异,及其对肝细胞癌增殖、迁移、侵袭、上皮细胞间质化(EMT)的影响.方法 2018年9月—2020年6月于西安交通大学实验室进行细胞实验,使用qRT-PCR分别检测92例经手术切除的肝癌组织与对应癌旁肝组织,肝癌细胞HepG2...

Study on the role of long non-coding RNA TPT1-AS1 in promoting the proliferation,migration and invasion of liver cancer cells

Objective To study the difference in expression of tumor protein translation regulator 1 antisense RNA1(TPT1-AS1)in hepatocytes and hepatocarcinoma cells,and its effects on hepatocellular carcinoma proliferation,migration,invasion,and epithelial cell interstitialization(EMT).Methods From September 2018 to June 2020,cell experiments were carried out in the Laboratory of Xi'an Jiaotong University,using qRT-PCR to detect 92 cases of surgically resected liver cancer tissues and corresponding adjacent liver tissues,liver cancer cells HepG2,SNU-182 and human liver immortalized cells The relative expression of TPT1-AS1 in THLE-3.According to the median of TPT1-AS1 expression in liver cancer tissue measured by qRT-PCR(3.38),patients were divided into TPT1-AS1 high expression group(n=46)and low expression group(n=46).Kaplan-Meier method was used to analyze and compare the difference in 5-year survival rate between the two groups.HepG2 and SNU-182 cells were transfected with siRNA to silence TPT1-AS1 expression,and the same cells were transfected with negative control siRNA(si-NC)as a control.Compare the proliferation,migration,invasion and EMT ability of siRNA and si-NC transfected HepG2 and SNU-182 cells.Results Compared with the corresponding adjacent tissues,the relative expression level of TPT1-AS1 in liver cancer tissues increased(t/P=54.065/0.000).The proportion of BCLC stage C and lymph node metastasis were higher in the high expression group(χ²/P=15.187/0.001,7.379/0.007).The 5-year survival rate of patients in the high expression group was lower than that in the low expression group(χ²/P=7.354/0.007).Compared with the corresponding cells transfected with si NC,the number of cell colonies in HepG2 and SNU-182 cells transfected with siRNA was significantly reduced,the ratio of cells in G0/G1 phase was increased(P=0.000),and the ratio of cells in S phase was significantly reduced(P=0.000),The relative migration distance is reduced(t/P=25.153/0.000),the number of cells penetrating the basement membrane is reduced,the G1/S phase transition and the relative expression of EMT-related proteins CDK4,N-cadherin and Vimentin are reduced(P=0.000),While the relative expression of p21 and E cadherin protein increased(P=0.000).Conclusion The long non-coding RNA TPT1-AS1 promotes the proliferation,migration,invasion and EMT of liver cancer cells.TPT1-AS1 may be used as a therapeutic target for hepatocellular carcinoma.