Technical Modifications of the Orthotopic Lung Transplantation Model in Rats With Brain-Dead Donors |
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| Authors: | Norberto Santana Rodríguez Pedro Llontop Santisteban Ana López García Bernardino Clavo Miguel Ángel Ponce González Juan Carlos Rodríguez Bermejo José Manuel García Castellano Ricardo García Herrera Keila Zerecero José Antonio Ruíz Caballero Yanira Brito Godoy Andrés Varela De Ugarte |
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| Affiliation: | 1. Departments of Pathology and Pediatrics, Stanford University School of Medicine;2. Department of Pathology and Laboratory Medicine, Emory University School of Medicine;1. Environment Canada, Watershed Hydrology and Ecology Research Division, Centre Saint-Laurent, 105 McGill St., Montreal, QC H2Y 2E7, Canada;2. Agriculture and Agri-Food Canada, Science and Technology Branch, Saskatoon, SK S7N 0X2, Canada |
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| Abstract: | BackgroundMicrosurgical lung transplantation in rats has allowed us to obtain new knowledge about lung transplantation. However, some aspects in human transplantation technique still have not been included in this model, which could interfere with the clinical interpretation and extrapolation of results.MethodsTwenty left lung transplantations were performed with a cuff technique and technical modifications, such as brain death induction, the control of ischemia time and retrograde perfusion in the donor and the controlled sequential reperfusion of the implanted lung in the recipient.ResultsSurvival rate was 80%. The transplanted lungs showed proper perfusion and ventilation with good permeability of the anastomoses. Signs of ischemia-reperfusion injury were observed in all animals while mild acute rejection was seen in half of them.ConclusionsThe shown model proves valid and is very similar to the procedure carried out in humans, which would reduce the number of possible variables derived from the surgical technique when extrapolating the study results to clinical use. |
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