| hsa_circ_0003188对人骨髓间充质干细胞成骨分化能力的影响 |
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| 引用本文： | 梁甲武,胡巍,屈爽,袁一方,郭斌. hsa_circ_0003188对人骨髓间充质干细胞成骨分化能力的影响[J]. 实用口腔医学杂志, 2021, 0(2): 207-211 |
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| 作者姓名： | 梁甲武 胡巍 屈爽 袁一方 郭斌 |
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| 作者单位： | 解放军医学院研究生院;中国人民解放军总医院第一医学中心口腔科 |
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| 基金项目： | 国家自然科学基金(编号:81470754)。 |
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| 摘 要： | 目的:探讨hsa_circ_0003188对人骨髓间充质干细胞(hBMSCs)成骨分化能力的影响.方法:Human Circular RNA Micro-array Version 2.0芯片检测正常环境与炎性环境(10 ng/mL TNF-α)培养的hBMSCs差异表达circRNAs,并筛选出目的circRNA-h...
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| 关 键 词： | 环状RNA hsa_circ_0003188 人骨髓间充质干细胞 炎性环境 成骨分化 |
The effects of hsa_circ_0003188 on osteogenic differentiation of bone marrow mesenchymal stem cells in inflammatory environment |
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| LIANG Jiawu,HU Wei,QU Shuang,YUAN Yifang,GUO Bin. The effects of hsa_circ_0003188 on osteogenic differentiation of bone marrow mesenchymal stem cells in inflammatory environment[J]. Journal of Practical Stomatology, 2021, 0(2): 207-211 |
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| Authors: | LIANG Jiawu HU Wei QU Shuang YUAN Yifang GUO Bin |
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| Affiliation: | (Graduate School of Chinese PLA Medical School,Beijing 100853 China;Department of Stomatology,the First Medical Center,Chinese PLA General Hospital,Beijing) |
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| Abstract: | Objective:To investigate the effects of hsa_circ_0003188 on osteogenic differentiation of human bone marrow mesenchymal stem cells(hBMSCs).Methods:Human Circular RNA Microarray Version 2.0 microarray was used to detect the differentially expressed circRNAs in hBMSCs cultured in normal and inflammatory environment(10 ng/mL TNF-α)respectively and to screen out the target circRNA-hsa_circ_0003188.After Lent-hsa_circ_0003188 transfection into hBMSCs and osteogenic induction,the effect of high expression of hsa_circ_0003188 on osteogenic differentiation of hBMSCs was detected by alkaline phosphatase staining.TargetScan and other biological softwares were used to predict the biological function of hsa_circ_0003188.Results:hsa_circ_0003188 was highly expressed in hBMSCs in inflammatory environment and Lent-hsa_circ_0003188 inhibited the osteogenic differentiation of hBMSCs.Biology softwares predicted the ceRNA mechanism of hsa_circ_0003188.Conclusion:hsa_circ_0003188 can inhibit the osteogenic differentiation of hBMSCs,which may become a new regulatory target for hBMSCs during bone regeneration. |
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| Keywords: | Circular RNA hsa_circ_0003188 Human bone marrow mesenchymal stem cells Inflammatory environment Osteogenic differentiation |
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