| HPLC法测定华山参滴丸中东莨菪碱、阿托品及东莨菪内酯的含量 |
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| 引用本文： | 周军,赵晨,张洋,张红意,曲佳,王杰.HPLC法测定华山参滴丸中东莨菪碱、阿托品及东莨菪内酯的含量[J].药物分析杂志,2012(5):892-894,918. |
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| 作者姓名： | 周军 赵晨 张洋 张红意 曲佳 王杰 |
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| 作者单位： | 天津市药品检验所;天津中新药业集团达仁堂制药厂;天津中新药业集团有限公司第六中药厂 |
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| 摘 要： | 目的:建立HPLC法测定华山参滴丸中东莨菪碱、阿托品及东莨菪内酯的含量。方法:采用资生堂CAPCELL-PAK C18(4.6 mm×250 mm,5μm)色谱柱,以乙腈-甲醇-磷酸氢二钠缓冲液(15∶4∶81)为流动相,流速1.0 mL·min-1,东莨菪碱、阿托品检测波长210 nm(0～15 min),东莨菪内酯检测波长为344 nm(15.1～30 min),柱温30℃。结果:东莨菪碱、阿托品及东莨菪内酯的线性范围分别为0.04～3.95,0.04～4.10,0.05～0.54μg(r=0.9999);平均回收率(n=6)分别为97.58%(RSD=1.3%),98.65%(RSD=1.3%),98.42%(RSD=1.8%)。结论:本文方法简便、准确,可以用于华山参滴丸中东莨菪碱、阿托品及东莨菪内酯的含量测定。
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| 关 键 词： | 华山参滴丸 止咳平喘药 托品烷类生物碱 东莨菪碱 阿托品 东莨菪内酯 高效液相色谱法 多成分分析 质量控制 中药制剂 标准提高 |
RP-HPLC analysis of scopolamine,atropine andscopoletin in Huashanshen dripping pills |
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| ZHOU Jun,ZHAO Chen,ZHANG Yang,ZHANG Hong-yi,QU Jia,WANG Jie.RP-HPLC analysis of scopolamine,atropine andscopoletin in Huashanshen dripping pills[J].Chinese Journal of Pharmaceutical Analysis,2012(5):892-894,918. |
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| Authors: | ZHOU Jun ZHAO Chen ZHANG Yang ZHANG Hong-yi QU Jia WANG Jie |
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| Institution: | 1(1.Tianjin Institute of Drug Control,Tianjin 300070,China;2.Tianjin Zhongxin Pharmaceutical Group Corporation LTD,Darentang Traditional Chinese Medicine Factory,Tianjin 300457,China;3.Tianjin Zhongxin Pharmaceutical Group Corporation LTD,No.6 Traditional Chinese Medicine Factory,Tianjin 300401,China) |
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| Abstract: | Objective:To establish an HPLC method for determination of scopolamine,atropine and scopoletin in Huashanshen dripping pills.Methods:The analysis was carried out on a CAPCELL-PAK C18(4.6 mm×250 mm,5 μm)column;The mobile phase was composed of acetonitrile-methanol-disodium hydrogen phosphate buffer solution(15∶4∶81)and the flow rate was 1.0 mL·min-1;The detection wavelengths were set at 210 nm for scopolamine and atropine(0-15 min),and 344 nm for scopoletin(15.1-30 min);The column temperature was 30 ℃.Results:The linear ranges of scopolamine,atropine and scopoletin were 0.04-3.95,0.04-4.10,0.05-0.54 μg(r=0.9999),respectively;The average recoveries(n=6)were 97.58%(RSD=1.3%),98.65%(RSD=1.3%),and 98.42%(RSD=1.8%),respectively.Conclusion:This method is simple,and can be used to determine the above three components with satisfactory accuracy and repeatability. |
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| Keywords: | Huashanshen dripping pills antitussive and antiasthmatic drugs tropane alkaloids scopolamine atropine scopoletin HPLC multicomponent assay quality control TCM preparation standard improvement |
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