大鼠实验性胃溃疡自愈期间胃黏膜三叶因子2的变化

目的 探讨大鼠实验性胃溃疡自愈期间胃黏膜三叶因子2(TFF2)的表达变化. 方法 胃前壁黏膜下注射冰乙酸,制备大鼠胃溃疡模型,免疫组织化学法及RT-PCR法分别从蛋白水平及基因水平检测正常组(6只)、溃疡组(42只)和盐水组(42只)大鼠胃黏膜组织TFF2的表达变化. 结果 免疫组织化学结果显示,正常组大鼠胃黏膜TFF2蛋白呈弱阳性表达.溃疡术后1d组TFF2阳性细胞即显著增多,至溃疡术后6d TFF2积分吸光度值达到高峰,阳性细胞以靠近黏膜肌层信号较强,壁细胞阳性表达较多.溃疡术后10d及14d TFF2积分吸光度值低于溃疡术后6d组,但仍维持于较高水平;溃疡术后23d TFF2积分吸光度值显著降低,但高于对照组(P<0.05或P<0.01).RT-PCR结果显示,TFF2 mRNA变化趋势与免疫组织化学结果基本一致,以溃疡术后4d和6d表达较为强烈. 结论 TFF2在维持胃黏膜的完整性和促进其快速修复过程中起重要作用.

Expression of trefoil factor family-2 in gastric mucosa during healing of experimental gastric ulcer in rats

Objective To explore the expression of trefoil factor family 2(TFF2) in gastric mucosa during healing of experimental gastric ulcer of rats. Methods Acetic acid induced ulcers were produced by injection acetic acid to submucosa of gastric anterior wall. The expressions of TFF2 peptide/mRNA in gastric ulcer group(GU,42 adult male rats), normal salinel group(NS,42 adult male rats,) and normal group(N,6 adult male rats) were detected by immunohistochemistry (IHC) and RT-PCR respectively. Results The results of IHC showed that the expression of TFF2 peptide in N group was weakly positive, and on the day 1 after acetic acid injection, TFF2 positive cells significantly increased,and reached the peak on the day 6TFF2 showed more stronger expression near muscularis mucosa,which mainly located in the cytoplasm of parietal cells. On the day 10 and 14 after acetic acid injection, the integral absorbance(IEM>A/EM>) values of TFF2 were lower than that on the day 6, but maintained a higher expression.On the day 23,the expression of TFF2 in GU group significantly decreased,but was more than that in the control groups(EM>P/EM><0.05 or EM>P/EM><0.01).The result of RT PCR showed that the change of TFF2 mRNA was consistent with TFF2 peptide,TFF2 mRNA showed more stronger expression on the day 4 and 6 in GU group. Conclusion TFF2 plays