钩端螺旋体C-70培养基的研制

本文报道C-70培养基对四个型的钩端螺旋体菌种的培养与检定。所培养的菌种,形态正常,运动活泼。以1％种子量(菌液浓度10亿/ml)接种时,培养7天,即能达到生长高峰期。生长浓度比原4·1培养基提高4.5～11.6倍(由原来1.5～2亿/ml提高到10亿/ml左右)。反向炭凝试验测定钩体抗原效价也高出4～64倍(由原来1:128～256++提高到1:1024～8196++)。制成菌苗后,物理性状良好,安全试验符合钩体菌苗制检规程合格标准,抗原经30倍稀释(含菌0.33亿/ml)作保护力试验,免疫动物100％保护。

Deve lopment of C-70 Medium for Leptospira

In this study, a new chemically-defined medium, C-70 medium prepared by us is used for the cultivation and identification of 4 serovars of ieptos-piras. Leptospiras grown in this medium all had typical shape and active movement. If amount of leptospiral inoculation was about 1% of the volume of C-70 medium, the maximum density of culture would appear on the 7th day after the incubation. The concentration of leptospiral growth in C-70 medium ( 108/m 1) was higher than in 4·1 medium (1.5-2.0×107/ml ). The antigen titer of leptospiras examined by reversed carbon-agglutination test also increased 4-64 times. The vaccine prepared with C-70 medium is sale and it's physical property is good. The antigen diluted in 1:30(0.33×107/ml) could protect 100% immunized animals against leptospirosis.