贲门肠上皮化生组织中DAS-1蛋白表达与贲门癌的相关性研究

目的 探讨贲门肠上皮化生(IM)组织中DSA-1表达与贲门癌(GCA)发生的相关性.方法 采用免疫组化法分析65例GCA患者癌组织和距癌组织2 cm贲门IM组织、15例门诊患者贲门(齿状线下2 cm内)IM组织及25例门诊患者贲门炎性组织检测DAS-1蛋白表达.结果 GCA患者贲门IM组织中Ⅲ型占55.4%(36/65),显著高于门诊患者的13.3%(2/15,P<0.01).DAS-1蛋白在GCA癌组织中的表达率为78.5%(51/65),显著高于贲门IM组织[38.8%(30/80),P<0.01].从Ⅰ～Ⅲ型贲门IM组织中,DAS-1蛋白的表达率呈逐渐递增趋势,其中Ⅲ型为71.1%,显著高于Ⅰ型(0%,P<0.01).结论 DAS-1过表达的Ⅲ型贲门IM与GCA的关系密切,可能是GCA癌前病变的重要因素.

Relationship between expression of DAS-1 protein in intestinal metaplasia and gastric cardia adenocarcinoma

Objective To investigate the relationship between expression of DAS-1 in gastric cardia intestinal metaplasia(CIM)and gastric cardia adenocarcinoma (GCA). Methods The cancerous tissues and CIM tissues (2 cm apart from caneer) obtained from 65 patients with GCA were examined for the expression of DAS-1 protein using immunohistoehemistry. The CIM tissues (<2 cm below Z line) obtained from 15 outpatients and inflammatory mucosa from 25 outpatients were also examined for expression of DAS-1 protein. Results The type Ⅲ IM was accounted for 55.4% (36/65) in GCA patients, which was significantly higher than that in outpatients [13.3% (2/15), P<0.01]. The positive rate of DAS-1 expression in cancerous tissues [78.5 % (51/65)] was also significantly higher than that in CIM tissues [38.8 %(30/80), P<0.01]. The expression of DAS-1 protein in IM tissues was gradually increased from type Ⅰ (0%) to type Ⅲ (71.1%) with positive correlation (P<0.01). Conclusions The type Ⅲ IM with over-expression of DAS-1 is closely related to GCA, which might be one of important precancerous lesions for GCA.