内皮祖细胞对骨髓间充质干细胞－种植体复合物成骨能力的影响

目的：研究内皮祖细胞（EPCs）对骨髓间充质干细胞（BMSCs）膜片－种植体复合体成骨能力的影响。方法：分离培养大鼠 BMSCs 并采用细胞膜片技术培养细胞膜片；分离培养大鼠骨髓来源 EPCs；将 EPCs 加载于 BMSCs 细胞膜片上并检测相关基因；制作细胞膜片－种植体复合体并植入裸鼠皮下，8周取材，行 Micro-CT 检查及组织学检测。结果：体外实验显示EPCs 加载于 BMSCs 细胞膜片后成骨相关 Runx-2、ALP、BMP2和成血管相关 VEGF 的基因表达提高；裸鼠体内实验取材 Mi-cro-CT 检查显示加载 EPCs 组成骨量较高，HE 切片显示加载 EPCs 组成骨面积较多，免疫组化检测显示加载 EPCs 组 Runx2、BMP2表达较高。结论：加载 EPCs 能提高 BMSCs-种植体复合物的成骨能力。

The impact of endothelial progenitor cells on the osteogenesis of bone marrow mesenchymal stem cell sheet-implant complex

Objective:To study the effects of endothelial progenitor cells(EPCs)on the osteogenesis of bone marrow mesenchymal stem cell (BMSCs)sheet-implant complex.Methods:EPCs were added to the BMSC sheets,and the expression of osteogenesis-relat-ed genes was examined by real time PCR.Cell sheets were wrapped around implants to construct cell sheet-implant complexes and the complexes were subcutaneously transplanted into SCID mice.The complexes were harvested 8 weeks after operation and observed by micro-CT and histological examination.Results:The BMSC sheet with EPCs showed higher expression of Runx2,ALP,BMP2 and VEGF in the in vitro test;higher bone volume ratio,greater amount of new bone tissue and higher expression of Runx2 and BMP2 in the in vivo test.Conclusion:EPCs can improve the osteogenesis of BMSC sheet-implant complex.