CKIP-1对骨髓间充质干细胞体外增殖和分化能力的影响

目的：探讨CKIP-1基因对小鼠骨髓间充质干细胞（BMSCs）的增殖和分化能力的调控。方法：选择CKIP-1基因敲除型小鼠（KO）和野生型C57小鼠（WT），采用全骨髓贴壁法培养BMSCs，取第3代BMSCs，分为KO组和WT组，分别采用成骨及成脂诱导液对细胞进行诱导培养，MTT法检测细胞增殖，流式细胞仪检测目的细胞表面标记分子，用ALP染色、茜素红染色、油红O染色分别对细胞成骨及成脂能力做定量分析。结果：2种细胞增殖和干细胞分子表达相似；成骨诱导后ALP染色显示，KO组的细胞染色的阳性率要大于WT组。茜素红染色观察显示KO组的矿化结节要多于WT组；油红O染色显示KO组细胞的脂质沉淀量大于WT组。结论：CKIP-1基因缺失可以使BMSCs成骨及成脂分化能力增强，对其增殖影响不明显。

Regulation of the proliferation and differentiation of mice bone marrow mesenchymal stem cells by CKIP-1 in vitro

Objective:To investigate the regulation of proliferation and differentiation of bone marrow mesenchymal stem cells(BM-SCs)by CKIP-1 in vitro.Methods:BMSCs from CKIP-1 nock out(KO)and wild type(WT)C57 mice were isolated and cultured u-sing adherence method in vitro.BMSCs of the 3rd passage were induced to osteogenic and adipgenic differentiation.Cell proliferation was examined by MTT assay.Cell surface markers were tested by FCM.The osteogenic and adipogenic differentiation was studied by alkaline phosphatase (ALP)staining,alizarin red staining and oil red O staining.Results:The proliferation and cell marke expression of the 2 groups were similar.ALP staining of KO group was strong than that of WT group after osteogenic induction.Alizarin red stai-ning showed that there were more mineralized nodules in BMSCs of KO group than in those of WT group.Oil red O staining of KO mice BMSCs was stronger than that of WT.Conclusion:CKIP-1 deficiency can enhance the osteogenic and adipogenic differentiation without influence on the proliferation of BMSCs.