霉酚酸酯对系统性红斑狼疮患者免疫功能的影响

目的 观察新型免疫抑制剂霉酚酸酯（MMF）对系统性红斑狼疮（SLE）免疫功能的影响。方法 应用ELISA法、间接免疫荧光法和流式细胞术观察MMF、环磷酰胺（CTX）治疗前后SLE患者血清IL－6、IL－8、NTFα、sIL-2R、ANA、A－dsDNA及淋巴细胞亚群的变化以及MMF、CTX对造血系统、肝脏、肾脏功能的影响。结果 SLE患者经MMF治疗3个月后血清IL－6、IL－8、TNF－α、sIL-2R、ANA、A－dsDNA水平显著下降,CD3^ 、CD4^ 、CD4^ 、CD45RA^ 细胞显著增高,CD8^ 、CD4?^ CD45RO^ 、CD86 CD45RA^ 、CD8^ CD45RO^ 细胞显著减少。对造血系统、肝脏、肾脏功能无明显损害。结论 MMF可选择性地抑制T、B淋巴细胞,是一种治疗SLE毒副作用较少的新型免疫抑制剂。

Effect of mycophenolate mofetil on the immune function in patients with systemic lupus erythematosus

Objective　To observe the effect of mycophenolate mofetil (MMF),a new type of immunosuppressant,on the immune function in patiens with systemic lupus erythematosus (SLE).Methods　The changes of serum lL-6,IL-8,TNF-α,sIL-2,ANA,A-dsDNA and subsets of lymphocytes were observed in patients with SLE before and after treatment with MMF and CTX by ELISA,indirect immunofluorescent assay and flow cytometry,and the effect of MMF and CTX on the functions of hematopoiesis,liver and kidney were also observed.Results　Three months after treatment with MMF,the serum levels of IL-6,IL-8,TNF-α,sIL-2,ANA,and A-dsDNA in patients with SLE were significantly decreased,CD3＋,CD4＋ and CD4＋CD45RA＋ cells were significantly increased,and CD8＋,CD4＋CD45RO＋,CD8＋CD45RA＋,CD8＋CD45RO＋ cells were significantly decreased.No remarkable injury was observed on the functions of hematopoiesis, liver and kidney after treatment with MMF.Conclusion　MMF can inhibit T and B lymphocytes selectvely and is a new type of immunosuppresant to treat SLE with less toxicity and side-effect.