亚麻子提取物对D-半乳糖胺致小鼠急性肝损伤的保护作用

目的:探索亚麻子水提物和醇提物对D-半乳糖胺致小鼠急性肝损伤的保护作用。方法:采用D-半乳糖胺建立小鼠急性肝损伤模型。将90只小鼠分为9组,分别为空白组、模型组、联苯双酯滴丸组、亚麻子水提物和醇提物的低、中、高各3个剂量组。空白组和模型组每天ig等体积的生理盐水,其他各组按相应药物剂量每天ig1次,共10 d。第9天ig 12 h后,按600 mg·kg-1ip 10%D-半乳糖胺溶液进行造模。末次给药24 h后取小鼠眼球血,测定血清丙氨酸转氨酶(ALT),天冬氨酸转氨酶(AST)活性,测定肝组织中丙二醛(MDA)的含量和超氧化物歧化酶(SOD)活力、谷胱甘肽(GSH)的含量。取肝脏称重计肝指数,并切片,做HE染色,光镜观察肝脏病理组织学变化。结果:与正常组比较,模型组显著升高ALT,AST,MDA水平(P0.05,P0.01),降低SOD,GSH活性(P0.01),肝组织结构不清,肝细胞溶解坏死明显;与模型组比较,亚麻子水提物(高剂量组)和亚麻子醇提物(低剂量组)可增高血清中SOD活性(P0.05),降低MDA水平(P0.01),亚麻子治疗组均降低血清ALT,AST,降低肝指数,均有改善肝组织结构,减少肝细胞坏死数量,有肝细胞再生,高剂量组改善较明显。结论:亚麻子提取物对D-半乳糖胺致小鼠急性肝损伤有一定的保护作用。

Protective Effect of Lini Semen Extract on D-galactosamine Induced Acute Liver Injury in Mice

Objective: To explore the protective effect of Lini Semen water extract and alcohol extract on D-GaIN induced acute liver injury in mice. Method: The mice acute liver injury model was induced by D-GaIN.90 mice were divided into 9 groups, blank group, model group, Bifendate pills group, Lini Semen water extract and alcohol extracts, low, middle, high dose group. The blank group and the model group were given volume saline, the other groups were given corresponding drugs dose by gavage once daily for 10 d.At the ninth day of administration,according to the dosage of 600 mg · kg^-1 intraperitoneal injection of 10% D-GaIN amine solution was given toestablish the modle. 24 h after the last administration blood samples were collected to measure serum alanine aminotransferase (ALT) and aspartate aminotransferase (AST) activity.Malondialdehyde(MDA), glutathione(GSH), superoxide dismutase(SOD) in liver tissue and liver index were measured. The hepatic histological changes were observed by HE with light microscope. Result: Compared with the normal group, model group significantly increased ALT, AST, MDA levels (P <0.05, P <0.01), decreased SOD and GSH activity (P <0.01), hepatic tissue structure was not clear, dissolved necrosis of liver cells were obviously;observed.Compared with the model group, the Lini Semen water extraction high dose and alcohol extraction low dose groups could increase the activity of serum SOD (P <0.05), decrease the level of MDA (P <0.01). Lini Semen treatment group decrease serum ALT,AST and the liver index,and improved hepatic tissue structure, reduced the number of necrosis of liver cells, liver cell regeneration was observed.The high dose group improved obviously. Conclusion: Lini Semen extract has certain protective effect on acute liver injury induced by D-GaIN in mice.