威灵仙提取物对糖尿病肾病大鼠的作用

目的:研究威灵仙提取物对糖尿病肾病大鼠模型的治疗作用。方法:SD大鼠,随机分为正常组,模型组,卡托普利组(10 mg·kg-1),威灵仙提取物高、低剂量组(500,250 mg·kg-1),除正常组外,其余各组大鼠ip链脲佐菌素(STZ)制备成糖尿病大鼠模型,ig给药,1次/d,连续给药12周,观察威灵仙提取物对大鼠肾功能尿素氮(BUN),血清肌酸酐(SCr),肌酸酐清除率(CCr),尿白蛋白,尿白蛋白排泄率(UAER),肾肥大指数指标,空腹血糖(FBG),血脂甘油三酯(TG),总胆固醇(TC)和低密度脂蛋白胆固醇(LDL-C),高密度脂蛋白胆固醇(HDL-C)和氧化指标白细胞介素-6(IL-6),丙二醛(MDA),超氧化物歧化酶(SOD),谷胱甘肽过氧化物酶(GSH-Px)的影响,以及肾组织病理形态学的变化。结果:与正常组比较,模型组大鼠血清BUN,SCr,CCr,IL-6,尿白蛋白,UAER,肾肥大指数和FBG的水平明显升高,TG,TC和LDL-C的水平明显升高,HDL-C的水平明显降低,肾脏MDA含量明显升高,SOD和GSH-Px的水平明显降低,均具有明显统计学差异(P0.05,P0.01),病理组织显示肾小球基膜和肾小管增厚和膨胀;与模型组比较,威灵仙提取物明显降低糖尿病模型大鼠血清BUN,SCr,CCr,IL-6,尿白蛋白,UAER,肾肥大指数和FBG的水平(P0.05),降低TG,TC和LDL-C的水平(P0.05),升高HDL-C的水平(P0.05),降低肾脏MDA的含量(P0.05),升高SOD和GSH-Px的水平(P0.05),同时显著减轻了糖尿病大鼠肾小球基膜和肾小管增厚和膨胀的程度。结论:威灵仙提取物对糖尿病肾病引起的肾损伤有显著的抑制作用。

Effect of Extracts from Clematidis Chinensis Radix et Rhizoma on Diabetic Nephropathy in Rats

Objective: To study the effect of extracts from Clematidis Chinensis Radix et Rhizoma in treating diabetic nephropathy in rats. Method: Totally 50 SD rats were randomly divided into the normal group, the model group, the Captopril group (10 mg · kg^-1), Clematidis Chinensis Radix et Rhizoma extracts high and low dose groups (500, 250 mg · kg^-1), with 10 rats in each group. Except for the normal group, the rest groups were intraperitoneally injected with streptozotocin (STZ) to prepare the diabetic rat model and then orally given corresponding drugs, qd, for 12 weeks. The effect of extracts from Clematidis Chinensis Radix et Rhizoma on urea nitrogen(BUN), serum creationine(SCr), creatimine clearance rate(CCr), urinary albumin, urinar protein excretion rate(UAER), kidney hypertrophy index, fasting blood glucose, triglyceride(TG), total cholesterol(TC), low density lipoprotein cholesterol(LDL-C), high density lipoprotein cholesterol(HDL-C), interleukin-6(IL-6), malondialdehyde(MDA), superoxide dismutase(SOD), glutathion peroxidase(GSH-Px) and Renal tissue pathomorphological changes. Result: Compared with the model group, the model group showed notably higher serum BUN, SCr, CCr, IL-6, urinary albumin, UAER, kidney hypertrophy index and fasting blood glucose, TG, TC, LDL-c and MDA and lower HDL-C, SOD and GSH-Px, with notable statistical difference (P <0.05, P <0.01). In pathological tissues, renal glomerular basement membranes and renal tubule were thickened and swollen. Compared with the model group, extracts from Clematidis Chinensis Radix et Rhizoma showed remarkably lower BUN, SCr, CCr, IL-6, urinary albumin, UAER, kidney hypertrophy index and FBG (P <0.05), TG, TC and LDL-C (P <0.05), renal MDA content (P <0.05) and renal glomerular basement membrane and renal tubule thickening and swelling and higher HDL-C (P <0.05) and SOD and GSH-Px (P <0.05). Conclusion: Extracts from Clematidis Chinensis Radix et Rhizoma has a significant inhibitory effect on renal injury induced by diabetic nephropathy.