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| 人大肠癌细胞系HR 8348和HCe 8693 K－ras基因的点突变分析 | |
| 引用本文： | 罗建红,余应年. 人大肠癌细胞系HR 8348和HCe 8693 K－ras基因的点突变分析[J]. 中国病理生理杂志, 1994, 0(6) |
| 作者姓名： | 罗建红 余应年 |
| 作者单位： | 浙江医科大学医学分子生物学实验室 |
| 摘 要： | 本文采用修饰引物的聚合酶链式反应（ＰＣＲ）所建立的限制性片段长度多态性（ＲＦＬＰ），并结合ＰＣＲ直接测序方法，首次分析了我国建株大肠癌细胞系ＨＲ８３４８和ＨＣｅ８６９３的Ｋ－ｒａｓ基因点突变。发现该两细胞系均存在ｋ－ｒａｓ第１２密码子的点突变，其方式均为ＧＧＴ→ＧＡＴ；但未发现Ｋ－ｒａｓ第１３和６１密码子的突变。 |
| 关 键 词： | 肿瘤，癌基因，突变，多态现象 |
Analysis of k-ras point mutation in human colorectal cancer cell lines HR 8348 and HCe 8693 |
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| Luo Jian-Hong,Yu Ying-Nian. Analysis of k-ras point mutation in human colorectal cancer cell lines HR 8348 and HCe 8693[J]. Chinese Journal of Pathophysiology, 1994, 0(6) | |
| Authors: | Luo Jian-Hong Yu Ying-Nian |
| Abstract: | k-ras point mutation in human colorectal cancer cell lines HR 8348 and HCe 8639 were analyzed by an artificial restriction fragment length polymorphism (RF-LP) created by the polymerase chain reaction (PCR) with modified primers as well as PCR direct sequencing.It was found that a point mutation of k-ras codon 12 from GGT to GAT were present in both cell lines and no base change at codon 13 and 61 of k-ras gene were found. |
| Keywords: | Neoplasms Oncogenes Mutation Polymorphism |
| 本文献已被 CNKI 等数据库收录！ | |