| 十滴水高效液相色谱指纹图谱研究 |
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| 引用本文： | 谢鹏,王敏,郭琪. 十滴水高效液相色谱指纹图谱研究[J]. 华北国防医药, 2013, 0(11): 81-83 |
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| 作者姓名： | 谢鹏 王敏 郭琪 |
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| 作者单位： | [1]解放军白求恩国际和平医院保健科,石家庄050082 [2]北京军区总医院263临床部医务处,北京101149 [3]北京军区联勤部药品仪器检验所,北京100071 |
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| 基金项目： | 军队中医药科研专项课题（10ZYZ204） |
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| 摘 要： | 目的建立十滴水的高效液相色谱指纹图谱，为制剂质量控制提供可靠方法。方法选取10批次十滴水样品。色谱柱：AgilentSB—C18（4．6mm×250mm，5μm），流动相：甲醇-1％冰醋酸，梯度洗脱，检测波长：280nm，进样量：10μl，流速：1．0ml／min。结果时间窗宽度为0．5min，共标定了13个共有色谱峰，10批次十滴水的相似度良好。结论本方法简单可靠，可为提高十滴水的质量控制标准提供参考和依据。
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| 关 键 词： | 十滴水 指纹图谱 色谱法 高效液相 |
A Study on Fingerprint Chromatograms of Shidishui High.performance Liquid Chromatography |
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| XIE Peng,WANG Min,GUO Qi. A Study on Fingerprint Chromatograms of Shidishui High.performance Liquid Chromatography[J]. Medical Journal of Beijing Military Region, 2013, 0(11): 81-83 |
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| Authors: | XIE Peng WANG Min GUO Qi |
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| Affiliation: | 1. Department of Health Care, Bethune International Peace Hospital of PLA, Shiji- azhuang 050082, China; 2. Medical Department, 263 Clinical Department of General Hospital of Beijing Military Area Command, Beijing 101149, China; 3. Institute for Drug and Instrument Control, Combined Logistics Department of Bei- jing Military Area Command, Beijing 100071, China) |
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| Abstract: | Objective To establish fingerprint chromatogram of Shidishui high-performance liquid chromatogra- phy （HPLC） in order to provide a reliable method for the quality control of praeparatum. Methods Shidishui samples from 10 batches were collected. The chromatographic column was Agilent SB-C18 （4.6 mm × 250 mm, 5 μm） , the mobile phase consisted of methanol -1% glacial acetic acid with a gradient elution, at 280 nm with 10 μl of injection vol- ume for detection wavelength, and 1.0 ml/min of flow rate. Results The width of time window was set at 0.5min, and 13 common chromatographic peaks were marked. Shidishui from 10 batches had good similarity. Conclusion The meth- od is simple and reliable, and can provide a reference standard for the quality control of Shidishui. |
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| Keywords: | Shidishui Finger printing Chromatography, high performance liquid |
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