缺氧对人牙周膜成纤维细胞增殖和超微结构影响的实验研究

目的:体外培养人牙周膜成纤维细胞(HPLFS),观察缺氧对其生长增殖能力的影响.方法: 分离培养 HPLFS,随机分为 21% O2对照组和10%、5%、2% O2缺氧组,采用四唑盐(MTT)比色法检测 HPLFS增殖情况,透射电镜下观察细胞超微结构改变.结果:MTT 法检测,与对照组比,12 h、24 h,缺氧对细胞增殖随缺氧程度呈依赖性增强,但重度缺氧(2% O2)24 h组具有统计学差异;48 h、72 h,重度缺氧组细胞增殖与对照组相比明显降低,差别具有统计学意义.透射电镜下,重度缺氧24 h细胞胞质内粗面内质网和线粒体明显增多,细胞突起增多;72 h细胞发生退变,溶酶体增多.结论:长期重度缺氧条件下,牙周膜的改建和修复功能降低,可能是高原牙周疾病多发、牙周组织破坏较重的重要原因.

Experimental studies of hypoxia on the proliferation ability and ultrastructure of cultured human periodontal ligament fibroblasts

Objective: To observe the effect of hypoxia on changes of proliferation ability of cultured human periodontal ligament fibroblasts (HPLFS) in vitro. Methods: HPLFS were randomly divided into two groups (experimental group and control group) by different oxygen concentrations. The oxygen concentration of control group was 21%. The oxygen concentrations of experiment group were 10%, 5% and 2%. The proliferation ability of HPLFS was detected by MTT colorimetric assay. The cell ultrastructure was observed by transmission electron microscope (TEM). Results: MTT assay results showed that compared with the control group, at the 12 h and 24 h, cell proliferation was enhanced with the hypoxia degree. At 24 h, cell proliferation showed significant differences. At 48 h and 72 h, proliferation of the cultured HPLFS in severe hypoxia group reduced significantly. Observed by TEM, at 24 h, not only the number of mitochondria, rough endoplasmic reticulum but also cell process increased in the cultured HPLFS in severe hypoxia group. At 72 h, the number of lysosome increased and the cell structure degenerated. Conclusion: Long-time severe hypoxia may lower the repair and remodeling abilities of periodontium, which might be one of the important etiological factors of periodontal disease under condition of high altitude.