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| HPLC法测定血脂康胶囊中洛伐他汀的含量 | |
| 引用本文： | 谢静,李东,王敏,陈振德,原文鹏. HPLC法测定血脂康胶囊中洛伐他汀的含量[J]. 中国药房, 2010, 0(45): 4287-4289 |
| 作者姓名： | 谢静 李东 王敏 陈振德 原文鹏 |
| 作者单位： | 深圳市人民医院／暨南大学第二临床医学院药学部,深圳市518020 |
| 基金项目： | 广东省中医药局建设中医药强省科研课题（2060149）;深圳市科技计划（医疗卫生类）项目（200603267） |
| 摘 要： | 目的:建立以高效液相色谱法测定血脂康胶囊中洛伐他汀含量的方法。方法:色谱柱为Dionex-C18,流动相为甲醇-水(75:25),流速为1mL·min-1,柱温为22℃,检测波长为236nm,进样量为10μL;样品以75%乙醇超声提取10min后进样测定,以峰面积外标法进行定量计算。结果:洛伐他汀检测浓度线性范围为0.01183～0.4732mg·mL-1(r=0.9999),方法平均回收率为99.2%,RSD=1.8%(n=6)。结论:本方法简单、快速、准确,适用于测定血脂康胶囊中洛伐他汀的含量。 |
| 关 键 词： | 血脂康胶囊 洛伐他汀 含量测定 高效液相色谱法 |
Content Determination of Lovastatin in Xuezhikang Capsule by HPLC |
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| XIE Jing,LI Dong,WANG Min,CHEN Zhen-de,YUAN Wen-peng. Content Determination of Lovastatin in Xuezhikang Capsule by HPLC[J]. China Pharmacy, 2010, 0(45): 4287-4289 | |
| Authors: | XIE Jing LI Dong WANG Min CHEN Zhen-de YUAN Wen-peng |
| Affiliation: | (Dept. of Pharmacy, Shenzhen Municipal People＇s Hospital/Second Medical College of Jinan University, Shenzhen 518020, China) |
| Abstract: | OBJECTIVE： To establish an HPLC method for the content determination of lovastatin in Xuezhikang capsule. METHODS： A Dionex-C18 column was used with mobile phase consisted of methanol-water （75：25） at flow rate of 1 mL·min^-1. The detective wavelength was set at 236 nm. The column temperature was set at 22 ℃ and injection volume was 10 μL. The samples were extracted with 75% ethanol under ultrasonic condition for 10 min. External standard method was used. RESULTS： The linear range of lovastatin was 0.011 83-0.473 2 mg·mL^-1 （r=0.999 9） with an average recovery of 99.2% （RSD=1.8%, n=6）. CONCLUSION： The method is simple, rapid and accurate for the content determination of lovastatin in Xuezhikang capsule. |
| Keywords: | Xuezhikang capsule Lovastatin Content determination HPLC |
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