| 喉癌人乳头瘤病毒感染和p53蛋白表达的研究 |
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| 引用本文： | 秦兆冰 董明敏. 喉癌人乳头瘤病毒感染和p53蛋白表达的研究[J]. 临床耳鼻咽喉头颈外科杂志, 1997, 11(12): 546-549 |
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| 作者姓名： | 秦兆冰 董明敏 |
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| 作者单位： | 河南医科大学第一附属医院耳鼻咽喉科!郑州450052 |
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| 摘 要： | 对４４例喉鳞癌和１６例声带息肉患者采用原位核酸杂交技术探测其人乳头瘤病毒（ＨＰＶ）６Ｂ，１１，１６，１８型ＤＮＡ同源序列及ＬＳＡＢ免疫组化法探测其Ｐ５３蛋白的表达，结果：（１）喉癌与ＨＰＶ１６／１８杂交阳性率为４３．２％（１９／４４），声带息肉为１２．５％（２／１６）（Ｐ〈０．０５）（２）喉癌ｐ５３蛋白阳性率为５６．８％（２５／４４），声带息肉全部为阴性；（３）喉癌ＨＰＶ１６／１８杂交及ｐ５３蛋白
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| 关 键 词： | 喉鳞状细胞癌 人乳头瘤病毒 p53蛋白 原位杂交 |
Study on HPV infection and p53 protein expression in laryngeal carcinoma |
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| Qin Zhaobing, Dong Mingmin. Study on HPV infection and p53 protein expression in laryngeal carcinoma[J]. Journal of clinical otorhinolaryngology, head, and neck surgery, 1997, 11(12): 546-549 |
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| Authors: | Qin Zhaobing Dong Mingmin |
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| Affiliation: | First Affiliated Hospital, Henan Medical University, Zhengzhou. |
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| Abstract: | In order to evaluate the role of human papillomavirus (HPV) infection and p53 protein expression in laryngeal squamous cell carcinoma (LSCC), the in situ hybridization and the labelled streptavidin biotin (LSAB) immunohistochemical method were used to detect the presence of HPV genomes 6B, 11, 16, 18 and expression of p53 protein respectively in 44 specimens of LSCC. And 16 specimens of laryngeal polyp were selected as controls. RESULTS: 1. HPV genomes 16/18 were detected in 19 (43.2%) of 44 LSCCs and in 2 (12.5%) of 16 laryngeal polyps (P < 0.05). 2.25 (56.9%) of 44 specimens of LSCC were p53 protein positive whereas the laryngeal polyps were all p53 negative. 3. 12 (63.2%) of 19 HPV 16/18 DNA-positive specimens of LSCC showed p53 protein expression and 12 (48%) of 25 p53 protein positive specimens of LSCC showed HPV 16/18 infection. CONCLUSION: Expression of p53 protein and infection of HPV 16, 18 may play a role in carcinogenesis of LSCC, respectively or simultaneously. |
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| Keywords: | Laryngeal carcinoma Human papillomavirus p53 protein Insituhybridization Immunohistochemical method |
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