| 高效液相色谱法测定人血清中丙戊酸钠含量 |
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| 引用本文： | 展翔,徐玫. 高效液相色谱法测定人血清中丙戊酸钠含量[J]. 中国药业, 2009, 18(6): 25-26 |
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| 作者姓名： | 展翔 徐玫 |
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| 作者单位： | 1. 河南省郑州市中心医院,河南,郑州,450001
2. 河南大学药学院药物分析教研室,河南,开封,475001 |
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| 摘 要： | 目的建立测定人血清中丙戊酸钠浓度的高效液相色谱（HPLC）。方法将提取的血样用溴苯乙酮在一定条件下衍生化后进行色谱分析。采用Elite C18柱，流动相为甲醇-水（80：20），流速为1mL／min，柱温为室温，检测波长为235nm。结果丙戊酸钠的保留时间为8．14min，最低检出质量浓度为1．64μg／mL，平均回收率为98．37％，其他常用抗癫痫药物不干扰测定，在分析范围内具有良好的线性关系。结论该方法操作简便、快捷、准确，可满足临床快速检测的需要。
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| 关 键 词： | 高效液相色谱法 血药浓度 丙戊酸钠 药物监测 |
Determination of Sodium Valproate in Human Serum by HPLC |
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| Zhan Xiang,Xu Mei. Determination of Sodium Valproate in Human Serum by HPLC[J]. China Pharmaceuticals, 2009, 18(6): 25-26 |
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| Authors: | Zhan Xiang Xu Mei |
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| Affiliation: | 1. Zhengzhou Central Hospital of Henan Provincical, Zhengzhou, Henan, China 450001; 2. Department of Pharmaceutical Analysis, Pharmaceutical College of Henan University, Kaifeng, Henan, China 475001) |
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| Abstract: | Objective To establish a method for the determination of sodium valproate in human serum by HPLC. Methods The blood samples were analyzed by chromatography after derivation with bromoacetophenone under certain condition. The Elite C18 column was used with methanol-water（80：20） as the mobile phase and the flow rate of 1 mL/min. The temperature was 10-30 ℃, the wavelength was 235 nm. Results The minimal detectable concentration for sodium valproate was 1. 64 μg/mL. The retaining time of sodium valproate was 8.14 min. The average recovery rate was 98.37%. The other common antiepileptic drugs were not found to interfere with this chromatographic procedure. Conclusion This method is quick, simple, accurate, and suitable for clinical requirements of rapid detection. |
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| Keywords: | HPLC blood drug concentration sodium valproate drug monitoring |
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