不同吸附剂对肝衰竭患者血浆总胆红素、血氨及胆汁酸的吸附作用对比研究

目的比较几种吸附剂对肝衰竭患者血浆总胆红素、胆汁酸及血氨的吸附作用。方法收集4名肝衰竭患者血浆，将每位患者血浆分为6份，分别用以下6种吸附材料进行血浆灌注：丽珠HA330吸附树脂（HA330组）；用人血白蛋白包被的活性炭（活性炭＋HSA组）；AB-8大孔吸附树脂（AB8组）；用人血白蛋白包被经化学处理的AB-8大孔吸附树脂（化学处理的AB-8＋HSA组）；人血白蛋白包被乙基纤维素（EC＋HSA组）；用人血白蛋白包被经化学处理的乙基纤维素（化学处理的EC＋HSA组）。观察各组吸附剂对总胆红血素、胆汁酸和血氨的吸附效果并进行比较。结果除EC＋HSA组外其余各组对总胆红素、胆汁酸的吸附均有效（P〈0．05），其中化学处理的EC＋HSA组对总胆红素的吸附率最高（10．82±1．24）％，活性炭＋HSA组对胆汁酸吸附率最高（9．85±1．50）％；各组对血氨的吸附均有效（P〈0．05），化学处理的EC＋HGA组的吸附率最高（33．83±2．51）％。结论化学处理的EC＋HSA组对总胆红素、血氨、胆汁酸有较好的吸附效果。

Comparison study on the adsorption of bilirubin,bile acids and blood ammonia in plasma samples from live failure patients by different adsorbents

Objective To compare the adsorption of bilirubin, bile acids and blood ammonia in plasma samples from hepatic failure patients by different adsorbents. Methods We collected plasma samples from 4 hepatic failure patients, and divided each sample into 6 parts for 6 adsorbents： HA330 macroporous resin （HA330）, activated charcoal encapsulated with HSA （activated charcoal ＋ HSA）, AB-8 macroporous resin （AB-8）, AB-8 macroporous with hydroxy and diamine arms and coating with HAS （treated AB-8＋HSA）, ethyl cellulose coating with HAS （EC＋HSA） and ethyl cellulose with hydroxy and diamine arms encapsulated with HAS （treated EC＋HSA）. We then observed the adsorption of bilirubin, bile acids and blood ammonia in the plasma samples passed through the adsorbents. Results The adsorbents effectively adsorbed bilirubin and bile acids in the plasma samples （P〈0.05） except the adsorbent EC＋HAS. Among the adsorbents we used, treated EC＋HAS had the highest efficiency of bilirubin adsorption （10.82±1.24）%, and activated charcoal ＋ HSA showed the best ability to adsorb bile acids （9.85±1.50）%. All of the 6 adsorbents adsorbed blood ammonia （P 〈 0.05） with the highest capability using treated EC＋HSA （33.83±2.51）%. Conclusion Treated EC＋HSA is the best adsorbent for the adsorption of bilirubin, bile acids and blood ammonia.