顺铂对雌性大鼠骨密度影响的实验研究

目的本文通过对去势雌性大鼠的动物实验研究，观察顺铂对骨代谢、骨密度的影响，并探讨其可能机制。方法将三月龄雌性SD大鼠30只，随机分为对照组和化疗组，每组15只。各组大鼠均用1％戊巴比妥钠腹腔内麻醉，行双侧卵巢切除术。术后1W开始用药，每两周用药1次，共6次。对照组腹腔内注射生理盐水1ml；化疗组腹腔内注射顺铂2mg／kg溶于1ml生理盐水中。术后13W处死各组动物，处死前用代谢笼收集24h空腹尿标本，自动生化分析仪测定尿钙浓度并计算出24h排泌量。氯胺T氧化法测尿羟脯氨酸(HOP)浓度并计算出24h排泌量；麻醉动物，开腹后下腔静脉取血离心，放免法测骨钙素(BGP)、血尿素氮(BUN)；取腰椎L4-6椎体，双能X线骨密度测量仪测腰椎骨密度。结果化疗组与对照组相比，骨密度降低，24h尿HOP及24h尿Ca定量升高，均有显著意义，血BGP无差异。结论本实验表明顺铂对大鼠的骨代谢存在直接的不良影响，使骨吸收增加，表现为松质骨骨密度降低。

Effect of cisplatin on bone mineral density in female rats

Objective To investigate the effect of cisplatin (DDP) on bone metabolism and bone mineral density and its mechanism. Methods Thirty female SD rats of three months weighted 230-250g were randomly divided into 2 groups: (15 rats of each) , blank operated and operated and DDP treated groups. At the end of 13th week, the 24h urine was collected for measurement of urinary calcium (Ca), hydroxyproline (HOP) and osteocalcin (BGP) . Immediately after the animals were sacrifised, L4-6 vertebrae were dissected sacri-fised. Dual energy X-ray absorptiometry (DEXA) was used for measuring the bone mineral density (BMD) of L4-6 vertebrae. Results In the study group, the values of 24h urinary Ca and 24h urinary HOP was remarkably increased, but the values of BGP showed no significant difference between these groups. Conclusionts DDP has negative effect on BMD of lumbar vertebrae due to increased bone resorption.