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| PEDF对大鼠视网膜血管内皮细胞迁移及VEGF表达的影响 | |
| 引用本文： | 张杰,杨旭东,申梅淑. PEDF对大鼠视网膜血管内皮细胞迁移及VEGF表达的影响[J]. 牡丹江医学院学报, 2010, 31(2): 9-10 |
| 作者姓名： | 张杰 杨旭东 申梅淑 |
| 作者单位： | 牡丹江医学院,黑龙江,牡丹江,157011 |
| 基金项目： | 牡丹江医学院科研项目(B20082) |
| 摘 要： | 目的：观察PEDF对体外培养的视网膜微血管内皮细胞迁移及VEGF蛋白表达的影响,初步探讨PEDF抗视网膜内皮细胞迁移的机制。方法：采用细胞划痕实验测定PEDF对糖尿病大鼠视网膜微血管内皮细胞迁移的影响;RT-PCR检测PEDF刺激后15,30,45,60min大鼠视网膜微血管内皮细胞的VEGF水平的变化。结果：PEDF对糖尿病大鼠视网膜微血管内皮细胞迁移具有抑制作用,且可抑制VEGF对糖尿病大鼠视网膜微血管内皮细胞促迁移作用,呈剂量依赖性。结论：PEDF抑制糖尿病大鼠视网膜微血管内皮细胞的迁移,其作用可能与VEGF水平有关。 |
| 关 键 词： | 细胞色素上皮衍生因子 视网膜微血管内皮细胞 迁移 |
THE EFFECT OF PEDF ON THE MIGRATION AND EXPRESSION OF VEGF IN RETINAL MICRO-VASCULAR ENDOTHELIAL CELLS |
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| Zhang Jie et al. THE EFFECT OF PEDF ON THE MIGRATION AND EXPRESSION OF VEGF IN RETINAL MICRO-VASCULAR ENDOTHELIAL CELLS[J]. Journal of Mudanjiang Medical College, 2010, 31(2): 9-10 | |
| Authors: | Zhang Jie et al |
| Affiliation: | Mudanjiang Medical College Mudanjiang Heilongjiang 157011 |
| Abstract: | Objective:To observe the effect of PEDF on the migration and expression of VEGF in retinal micro-vascular endothelial cells in rat.Methods:Cell scratch healing assay was used to examine PEDF interfering effect on the migration in retinal micro-vascular endothelial cells.The expression of VEGF was examined by RT-PCR in 15,30,45 and 60 minutes.Results:PEDF can inhibit the migration of cells directly and can inhibit the up-regulated migration of cells induced by VEGF.Conclusion:PEDF can inhibit the migration o... |
| Keywords: | PEDF retinal micro-vascular endothelial cells migration |
| 本文献已被 CNKI 维普 万方数据 等数据库收录！ | |