重组溶葡萄球菌酶对金黄色葡萄球菌生物被膜的体外清除作用

目的研究重组溶葡萄球菌酶对金黄色葡萄球菌生物被膜的体外清除作用。方法使用硅橡胶膜片建立金黄色葡萄球菌生物被膜的体外模型;使用超声震荡—活菌计数法作为金黄色葡萄球菌生物被膜的定量检测方法,分别测定在给予重组溶葡球菌酶及其对照药作用前后金黄色葡萄球菌生物被膜中的活菌数;使用扫描电镜作为金黄色葡萄球菌生物被膜的直观定性检测方法,分别观察在给予重组溶葡球菌酶及其对照药作用前后金黄色葡萄球菌生物被膜的镜下形态。结果经72 h连续培养,金黄色葡萄球菌在硅橡胶片上形成较为成熟的生物被膜,不同浓度重组溶葡球菌酶作用24 h后被膜中的活菌计数明显低于对照药去甲万古霉素,电镜结果进一步支持此结果。结论重组溶葡萄球菌酶能够有效地清除金黄色葡萄球菌生物被膜。

In vitro eradication of S.aureus biofilms by recombinant lysostaphin

Objective To investigate the in-vitro eradication of S. aureus biofilm by recombinant lysostaphin. Methods The in-vitro biofilms of S. aureus were established on silica gel plates. The biofilms were then treated with recombinant lysostaphin or norvancomycin, compared with those untreated as controls. Before and after treatment, ultrasonic viable bacterial count as a measure of quantitative assessment was evaluated in these biofilms. Scanning elec- tron microscopy （SEM） was employed to study the morphology of bacterial biofilms with or without treatment. Results After continuous cultivation for 72 hours, mature biofilms of S. aureus were found to form on silica gel plates. The viable bacterial counts of biofilm after 24h incubation with recombinant lysostaphin were obviously lower than those with norvancomycin. This finding was further supported by subsequent SEM. Conclusion Recombinant lysostaphin appeared useful for effective eradication of S. aureus biofilm.