水灾后浙江省余姚市主城区河水沙门菌检测与分析

目的 了解浙江省余姚市主城区河水中沙门菌污染情况、血清分型及药物敏感情况。方法 采集余姚市主城区12个监测点河水，进行增菌、分离培养、生化鉴定、血清分型及药敏试验。结果 110份河水中有58份河水检出沙门菌，阳性率52.73%；共检出84株沙门菌，总检出率为76.36%。分为17种血清型，优势菌为鼠伤寒沙门菌（34.52%）和德尔卑沙门菌（16.67%）。姚江三管堂、候青江原家禽医院污染率最高，污染种类最多。对头孢类、单环-内酰胺类、-内酰胺/-内酰胺酶抑制剂、喹诺酮类、氨基糖苷类敏感率较高，对氨苄西林、复方新诺明、哌拉西林、妥布霉素、庆大霉素耐药率分别为67.86%、53.57%、51.19%、20.24%、17.86%。耐药菌65株（77.38%），多重耐药菌18株（21.43%），其中3株沙门菌耐10种以上抗生素。结论 余姚市主城区河水沙门菌污染率高，菌型繁多，耐药率高；优势菌是鼠伤寒沙门菌和德尔卑沙门菌，与人源感染优势菌血清型一致。

Detection of Salmonella in river in urban area of Yuyao after flood

Objective To understand the contamination, serotyping and antibiotic susceptibility of Salmonella in the rivers in urban area of Yuyao, Zhejiang province. Methods The samples were collected from 12 monitoring sites in Yuyao, and then enrichment, isolation, biochemical identification,serotyping and drug susceptibility test of the isolates were carried out. Results Salmonella was detected in 58 of 110 river water samples (52.73%), and 84 strains of Salmonella were detected (76.36%). The 84 strains were divided into 17 serotypes,the predominant serotype was Salmonella typhimurium,accounting for 34.52%, and Salmonella derby accounted for 16.67%.The contamination was most serious and complex in Sanguantang of Yaojiang river and in poultry hospital of Houqingjiang river.These strains had high sensitivities to cephalosporins and monobactams, beta lactam/beta lactamase inhibitors, quinolones and aminoglycosides, and the resistant rate to ampicillin, cotrimoxazole, piperacillin, tobramycin and gentamicin were 67.86%,53.57%,51.19%,20.24%and 17.86% respectively. It was found that 65 strains were drug resistant,accounting for 77.38%, and 18 strains were multidrug resistant, accounting for 21.43%,in which 3 strains were resistant to more than 10 antibiotics. Conclusion Salmonella contamination in the rivers in urban area of Yuyao was serious. The isolated strains had various serotypes and high drug resistant rate. The predominant serotype of Salmonella were Salmonella typhimurium and Salmonella derby which shared the same serotypes with those of Salmonella etected in human infections.