| 高效液相色谱法测定狼疮灵颗粒中芍药苷的含量 |
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| 引用本文： | 孙川,冯俭,李煌. 高效液相色谱法测定狼疮灵颗粒中芍药苷的含量[J]. 广州医药, 2010, 41(5): 56-58. DOI: 10.3969/j.issn.1000-8535.2010.05.028 |
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| 作者姓名： | 孙川 冯俭 李煌 |
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| 作者单位： | 成都中医药大学药学院,四川,成都,610072;成都中医药大学附属医院,四川,成都,610072 |
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| 摘 要： | 目的建立狼疮灵颗粒中芍药苷的高效液相色谱（HPLC）含量测定方法。方法采用HPLC法,色谱柱：Hypersil ODS-2（150 mm×4.6 mm,5.0μm）,流动相∶乙腈-0.4%的磷酸水溶液（10∶90）,检测波长∶230nm,流速：1.0ml/min,柱温：25℃。结果芍药苷在0.608－3.04μg范围内呈良好的线性关系（r=0.9999）,加样回收率97.77%,RSD为0.62%（n=5）。结论该方法快速可行、简捷准确、重现性好、结果可靠,可用于控制狼疮灵颗粒的质量。
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| 关 键 词： | 狼疮灵颗粒 芍药苷 高效液相色谱法 |
Determination of paeoniflorin in langchuangling granules by HPLC |
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| Sun Chuan,Feng Jian,Li Huang. Determination of paeoniflorin in langchuangling granules by HPLC[J]. Guangzhou Medical Journal, 2010, 41(5): 56-58. DOI: 10.3969/j.issn.1000-8535.2010.05.028 |
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| Authors: | Sun Chuan Feng Jian Li Huang |
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| Affiliation: | 1 Chengdu University of Traditional Chinese Medicine,610072,China.2 The Affilliated Hospital of Chengdu University of Chinese Medicine Hospitals,610072,China ) |
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| Abstract: | Objective To establish an accurate method for determination of Paeoniflorin in Langchuangling Granules.Methods Hypersil ODS-2（4.6mm×150mm,5.0μm） in an oven at 25℃ was need,with a mobile phase consisting of acetonitril-0.4% granolite treatment（10∶90）and UV detector at 230nm,the flow rate 1.0 ml/min.Results The linear range of Paeoniflorin was within 0.708～3.54μg（r=0.9999）.The average recovery was 97.77% and RSD was 0.62%（n=5）.Conclusion The method is simple and accurate.It can be used for the quality control of Langchuagnling Granules. |
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| Keywords: | Langchuangling Granules Paeoniflorin High performance liguid chromatography（HPLC） |
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