胃癌组织中Cyr61和核因子-κB的表达与转移及预后的关系

目的 检测胃癌组织中富含半胱氨酸肝素结合蛋白61(Cyr61)和核因子-κB(NF-κB)的表达及其与临床病理特征及预后的关系.方法 应用逆转录聚合酶链反应技术验证和检测53例胃癌组织与11例非肿瘤胃黏膜中Cyr61基因的表达,并用免疫组织化学方法 (SP法)检测99例胃癌组织与25例非肿瘤胃黏膜中cyr61与NF-κB蛋白的表达情况.结果 胃癌原发灶和转移灶中Cyr61mRNA的阳性表达率分别为84.6%(22/26)、88.9%(24/27),明显高于非肿瘤胃黏膜组5/11(P值均＜0.05).非肿瘤胃黏膜、原发癌与转移癌组的基因表达量(2-△Ct)分别为(2.76±5.50)×10-5、(14.61±20.64)×10-5、(18.46±26.38)×10-5.胃癌原发灶、转移灶Cyr61 mRNA均高于非肿瘤胃黏膜(P值均＜0.05),转移灶与原发灶比较,差异无统计学意义(P＞0.05).99例胃癌中Cyr61及NF-κB蛋白的高表达率分别为56.6%(56/99)和55.6%(55/99),其表达水平与肿瘤浸润深度、脉管侵犯、淋巴结转移、远处转移及TNM分期呈正相关(P值均＜0.05),NF-κB还与肿瘤大小呈正相关(P＜0.05);Cyr61蛋白的表达水平与NF-κB呈正相关(P＜0.05);Cyr61与NF-κB蛋白高表达的病例平均生存时间和5年生存率明显低于低表达的病例(P值均＜0.05).结论 Cyr61和NF-κB阳性表达与胃癌的侵袭、转移密切相关,检测Cyr61和NF-κB的表达可作为判断胃癌生物学行为的重要参考指标.

Relationship of cysteine-rich protein 61 and NF-κB expression with metastasis and prognosis of gastric carcinoma

Objective To investigate the expression of eysteine-rich protein 61 (Cyr61) and NF-κB in gastric carcinoma and its correlation with the clinieopathologic features and survival time.Methods RT-PCR was used to validate and detect expression of Cyrrl mRNA in 53 gastric carcinoma specimens and 11 non-tumor gastric mucosa samples.Cyr61 and NF-KB protein levels expressed were detected using immunohistochemistry in 99 gastric carcinoma specimens and 25 non-tumor gastric mueosa samples.Results RT-PCR demonstrated that expression of Cyr61 mRNA was higher in the primary carcinoma (84.6%,22/26) and the metastatic foei (88.9% ,24/27) than in the non-tumor control samples (5/11 ; P ＜0.05,respectively).Cyr61 gene mRNA expression levels were (2.76±5.50)×10-5,(14.61±20.64)×10-5,and (18.46±26.38)×10-5 by 2-△Ct in the control mucosa samples,primary carcinomas and metastatic tissues respectively.The level was higher in the primary carcinomas and metastatic tissues than that of the non-tumor gastric mueosa (P＜0.05,respectively); however,there was no significant difference between the metastatic tissues and the primary carcinomas (P＞0.05 ).Immunohistochemistry revealed that of the 99 cases,there was a high expression of Cyrrl and NF-κB protein,56.6% (56/99) and 55.6 % (55/99) respectively.There was correlation of Cyr61 and NF-κB protein expressions with the depth of tumor and vascular invasion,as well as the development of lymph node metastasis,and TNM staging (P＜0.05,respectively),besides,the expression of NF-κB also correlated with the tumor diameter (P＜0.05).Cyr61 expression was positively correlated with NF-κB expression in gastric carcinoma (P＜0.05) ; the mean survival time in cases with a high expression level of Cyr61 and NF-κB protein was significantly shorter than those with a low expression level (P＜0.05).Conclusions Expression of Cyr61 and NF-KB closely correlated with invasion and metastasis of gastric carcinoma.They may be considered as the biologic behavior indicators for gastric carcinoma.