| 黄芪联合鞘内移植间充质干细胞对大鼠缺氧缺血性脑损伤修复作用的研究 |
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| 引用本文： | 余勤,李佩佩,周丽萍,宣晓波,盛丽先,郭莹,林洁,董晖,王标. 黄芪联合鞘内移植间充质干细胞对大鼠缺氧缺血性脑损伤修复作用的研究[J]. 中华中医药学刊, 2011, 0(7): 1455-1458 |
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| 作者姓名： | 余勤 李佩佩 周丽萍 宣晓波 盛丽先 郭莹 林洁 董晖 王标 |
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| 作者单位： | 浙江中医药大学生物工程学院;浙江中医药大学第一临床医学院; |
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| 基金项目： | 浙江省卫生厅资助项目(2006A092); 浙江省新苗人才计划(2010R410046) |
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| 摘 要： | 目的:研究黄芪注射液联合鞘内移植大鼠骨髓间充质干细胞(rMSCs)对大鼠缺氧缺血性脑损伤(HIBD)修复的影响。方法:115只Wistar大鼠随机分成假手术组、模型组、PBS对照组、黄芪注射液对照组、rMSCs治疗组和rMSCs加黄芪注射液治疗组。造模后3天治疗组鞘内注射rMSCs,或同时腹腔注射黄芪注射液,对照组鞘内注射PBS或腹腔注射黄芪注射液,造模后24天行Morris水迷宫实验检测大鼠学习、记忆能力,移植后1、4、7、14、21、28天分别取脑组织作病理学检查,并应用双标免疫组化检测经5-溴-2-脱氧嘧啶(BrdU)标记rMSCs的神经元特异性烯醇化酶(NSE)和胶质纤维酸性蛋白(GFAP)表达情况。结果:治疗组学习、记忆能力较对照组有显著改善(P<0.05),rMSCs加黄芪注射液治疗组第3天平均逃避潜伏期显著低于rMSCs治疗组(P<0.05)。脑组织病理切片显示rMSCs加黄芪治疗组较rMSCs治疗组脑组织水肿、神经细胞变性和坏死程度减轻更明显。双标免疫组化显示rMSCs移植后1天就出现在脑组织,并表达NSE与GFAP。rMSCs加黄芪治疗组BrdU阳性细胞数除移植后14天外均显著多于rMSCs治疗组(P<0.05),NSE阳性率移植后1、7、14天显著高于rMSCs治疗组(P<0.05),GFAP阳性率除移植后14天外均显著高于rMSCs治疗组(P<0.05)。结论:鞘内移植rMSCs是治疗大鼠HIBD的有效方法;黄芪注射液在体内有诱导rMSCs向神经细胞分化的能力,并有协同rMSCs促进大鼠HIBD修复的作用。
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| 关 键 词： | 黄芪注射液 间充质干细胞 缺氧缺血性脑损伤 鞘内移植 |
Effect of Astrasalus Injection Combined with Intrathecal Transplantation of Rat Mesenchymal Stem Cells for Repairing Hypoxic-ischemic Brain Damage in Rats |
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| YU Qin,LI Pei-pei,ZHOU Li-ping,XU Xiao-bao,SHENG Li-xian,GUO Ying,LIN Jin,DONG Hui,WANG Biao. Effect of Astrasalus Injection Combined with Intrathecal Transplantation of Rat Mesenchymal Stem Cells for Repairing Hypoxic-ischemic Brain Damage in Rats[J]. Chinese Archives of Traditional Chinese Medicine, 2011, 0(7): 1455-1458 |
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| Authors: | YU Qin LI Pei-pei ZHOU Li-ping XU Xiao-bao SHENG Li-xian GUO Ying LIN Jin DONG Hui WANG Biao |
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| Affiliation: | YU Qin1,LI Pei-pei2,ZHOU Li-ping2,XU Xiao-bao2,SHENG Li-xian2,GUO Ying1,LIN Jin1,DONG Hui1,WANG Biao1(1.Bio-Engineering College,Zhejiang Chinese Medical University,Hangzhou 310053,Zhejiang,China,2.The First Clinical College,China) |
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| Abstract: | Objective:To observe the effect of astrasalus injection(ASI) combined with intrathecal transplantation of rat mesenchymal stem cells(rMSCs) for repairing hypoxic-ischemic brain damage(HIBD) in rats.Methods:115 Wistar rats were randomly divided into 6 groups,named respectively by letters from A-F.Excepting 14 rats in Group A were sham-operated,the other 101 were hypoxic-ischemic(H-I) insulted,which were treated respectively with nothing,PBS,ASI,rMSCs and ASI+rMSCs 3 days after H-I insult.Learning and memory ... |
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| Keywords: | astrasalus injection mesenchymal stem cells hypoxic-ischemic brain damage intrathecal transplantation |
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