临床常见念珠菌对抗真菌药物体外敏感性的研究

目的:研究念珠菌对6种抗真菌药物的敏感情况。方法:念珠菌收集2004年7月~2005年4月临床患者提供的标本,测定从临床血液、尿液、痰等标本中分离的300株常见念珠菌对两性霉素B、制霉菌素、氟康唑、咪康唑、克霉唑、酮康唑的敏感性。结果:白色念珠菌最常见占64.0%,其次为热带念珠菌占19.7%,光滑球拟酵母菌占11.7%,近平滑念珠菌占2.3%,其他念珠菌占2.3%。临床常见念珠菌对两性霉素B、制霉菌素、氟康唑敏感性最高,对唑类药物存在不同程度的耐药性。结论:应加强对念珠菌药物敏感性的检测,以指导临床抗感染治疗合理选择药物。

A Study on Susceptibility of Clinical Isolates Candida spp. to Antifungal Agents in vitro

Objective: To study the susceptibility of yeasts to six antifungal agents.Methods:The susceptibilities of 300 strains of Candida spp.clinically isolated from the blood,urine,sputum from July 2004 to April 2005 to Ampho,Nysta,Flucz,Ketoz,Micoz,Ctrim were determined by broth microdilution tests performed according to the National Committee for Clinical Laboratory Standards guideline(NCCLS M_(27)-A_2).Results: Strains of Candida albicans were the most frequent organism isolated which accounted 64.0% of all the isolates,C.tropicalis,C.parapsilosis and C.krusei accounted for 19.7%,11.7% and 2.3%.The others accounded for only 2.3%.The suscepfibilities of C.albicans,C.tropicalis,C.parapsilosis to Ampho,Nysta,Flucz were higher than that of other antifungal agents.There was the resistance of them to triazole antifugal agents.Conclusion:The surveillance of susceptibility of clinical isolates Candida spp.should be consfantly conducted,so as to help the doctors reasonably select the antifungal agents.