| 白细胞介素4基因转移的肿瘤细胞体外生物学特性及体内致瘤性的研究 |
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| 引用本文： | 于益芝 曹雪涛. 白细胞介素4基因转移的肿瘤细胞体外生物学特性及体内致瘤性的研究[J]. 中国免疫学杂志, 1995, 11(1): 26-30 |
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| 作者姓名： | 于益芝 曹雪涛 |
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| 作者单位： | 第二军医大学免疫学教研室 |
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| 基金项目： | 国家自然科学基金，军队八五攻关基金 |
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| 摘 要： | 观察了白细胞介素４（ＩＬ－４）基因转移的肿瘤细胞体内致瘤性的变化。构建了含５００ｂｐ的小鼠ＩＬ－４ｃＤＮＡ的真核表达载体ＢＭＧＮｅｏ－ＩＬ－４．用该表达载体将ＩＬ－４基因转移至小鼠Ｂ１６黑色素瘤细胞，通过Ｇ４１８抗性筛选、有限稀释法及ＩＬ－４活性检测，获得了高分泌ＩＬ－４的黑色素瘤细胞克隆株并经Ｎｏｒｔｈｅｒｎ印迹鉴定，将其接种于小鼠皮下后观察到肿瘤生长缓慢、小鼠存活期延长，表明高分泌ＩＬ－４的细胞克隆株体内致瘤性显著下降。该结果为进一步研究肿瘤的ＩＬ－４基因治疗打下了基础。
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| 关 键 词： | 白细胞介素4 基因转移 黑色素瘤 基因疗法 |
IN VITRO BIOLOGICAL CHARACTERISTICS AND IN VI-VO TUMORIGENICITY OF
TUMOR CELLS TRANSFECT-ED WITH INTERLEUN-4 GENE |
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| Yu Yizhi,Cao
Xuetao,Xu Zhigong et al. IN VITRO BIOLOGICAL CHARACTERISTICS AND IN VI-VO TUMORIGENICITY OF
TUMOR CELLS TRANSFECT-ED WITH INTERLEUN-4 GENE[J]. Chinese Journal of Immunology, 1995, 11(1): 26-30 |
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| Authors: | Yu Yizhi Cao
Xuetao Xu Zhigong et al |
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| Affiliation: | Yu Yizhi,Cao
Xuetao,Xu Zhigong et al .Department of immunology,second military
medical u-niversity,Shanghai 200433 |
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| Abstract: | he in vivo
tumorigenicity of melanoma cells engieered to secrete IL-4 was
observed.Theexpression vector BMGNeo-IL- 4 containing 500bp-long mIL-
4 cDNA was constructed and wastransfected into B16 melanoma cells.
The melanoma cell clones which secreted IL-4 were ob-tained after
G418 resistance selection ,limiting dilutiori and assay of IL-4
activity,and then anal-ysed by Northern blot. After the cell clones
were inoculated subvutaneously to mice,the tumorgrowth were inhibited
and the survival time of tumor-bearing mice was extended. The
resultsdemonstrated that the tumorigenicity of the melanoma cells
engineered to secrets IL-4 decreasedobviously compared with the
wild-type B16 cells. |
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| Keywords: | Interleukin 4 Gene transfer
Tumorigenicity Melanoma Gene therapy |
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