妊娠及非妊娠妇女宫颈感染人乳头瘤病毒的基因亚型检测及分析

目的:探讨妊娠及非妊娠妇女宫颈感染人乳头瘤病毒(human papilloma virus,HPV)的基因分型情况。方法:收集2012年2月—2013年5月在复旦大学附属金山医院妇产科门诊就诊的684例育龄期女性(非妊娠妇女)和857例妊娠妇女的宫颈上皮细胞标本,采用基因扩增及基因芯片技术对宫颈上皮细胞进行HPV基因亚型检测。结果:非妊娠妇女检出HPV感染195例(感染率28.5%),主要的感染类型为HPV43、16、58等。妊娠妇女检出HPV感染102例(感染率11.9%),主要的感染类型为HPV43、16、58等。妊娠妇女中30岁、30~40岁、40岁的HPV感染率分别为10.9%、15.1%、30%,非妊娠妇女中30岁、30~40岁、40岁的患者HPV感染率分别为25.3%、25.2%、32.2%。结论:妊娠妇女HPV感染率低于非妊娠妇女,两组HPV感染型别基本相同,HPV43、16、58型感染是该院妇产科门诊患者的主要感染型别,非妊娠妇女随年龄增长感染率明显升高。

Detection and Analysis on Gene Subtypes of Cervical Human Papilloma Virus Infection in Pregnant and Non-Pregnant Women

Objective：To investigate the gene subtypes of cervical human papilloma virus （HPV） infection in pregnant and non-pregnant women .Methods ：From February 2012 to May 2013 ,cervical epithelial cells in 684 women of childbearing age （non-pregnant women） and 857 pregnant women in Jinshan Hospital ,Fudan University were collected .Polymerase chain reaction （PCR） and gene-chip technique were used to detect HPV gene subtype .Results ：A total of 195 positive results were found in non-pregnant women with the HPV infection rate of 28 .5% .The predominant types of HPV infection were type 43 ,16 and 58 .A total of 102 positive results were found in pregnant women with the HPV infection rate of 11 .9% .The predominant types of HPV infection were type 43 ,16 and 58 .The infection rates of pregnant women in less than 30 years of age ,30 to 40 years old ,more than 40 year old were 10 .9% ,15 .1% and 30% ,respectively .The infection rates of non-pregnant women in less than 30 years ,30 to 40 years old ,more than 40 year old were 25 .3% ,25 .2% and 32 .2% ,respectively .Conclusions ：The HPV infection rate of pregnant women was lower than that of non-pregnant women .The main HPV infection subtypes of the pregnant women and the non-pregnant women were the same .HPV43 ,16 and 58 were the predominant subtypes in this hospi-tal .The infection rate of non-pregnant women increased significantly in accordonce with age .