OPN与MMP-9在大鼠子宫内膜异位症中的表达及意义

目的探讨OPN与MMP-9在大鼠内异症模型异位内膜中表达的特点及OPN与MMP-9的相关因子。方法大鼠手术建模28d后二次手术开腹,肉眼观合并组织病理学检查确定建模成功后,取大鼠的在位（在位组）、异位内膜组织（异位组）和建模前在位内膜组织（对照组）,通过RT-PCR、免疫组化法测定内膜中OPN与MMP-9 mRNA及蛋白表达。结果 OPN表达于腺上皮细胞的细胞膜上,间质内少量表达;MMP-9主要表达于腺上皮细胞的胞浆内,核内无表达;异位内膜组中OPN表达的积分光密度值（IOD）为53 735.4±4 896,高于在位内膜组33 733.8±5 322和对照组（37 291.2±4 588,P〈0.05）,对照组和在位组的IOD值差异无统计学意义（P〉0.05）;免疫组化异位组内膜中MMP-9的表达为32 645±9 967,高于在位内膜组和对照组（P〈0.05）,而在位组和对照组两组比较差异无统计学意义（P〉0.05）;OPN与MMP-9 mRNA的表达呈正相关关系。结论 OPN与MMP-9在大鼠子宫内膜异位症模型中呈正相关,两者在细胞信息调节中可能存在级联调控关系。

The expression and significiance of OPN and MMP-9 in endometriosis rats

Objective To analyze the expression of OPN and MMP-9 in ectopic endometrium and the relationship between OPN expression and MMP-9 in endometriosis.Methods The ectopic endometrial tissue（ectopic group） and eutopic endometrium from 37 pre-built model rats was compared with former model（control group） in order to determinate of the expression of OPN and MMP-9mRNA and protein.Results OPN highly expressed in the plasma membrane of epithelial cellsand a small amount of expression of interstitial while MMP-9 was mainly expressed in the cytoplasm of glandular epithelial cells and no expression in the nucleus;Immunohistochemistry showed that OPN expression in ectopic endometrium IOD（53 735.4±4 896）.And it was significantly higher than that in eutopic group（33 733.8 ± 5 322） and a control group（37 291.2±4 588）.Control group and the reign of group no significant difference in IOD（P〉0.05）;The IOD of MMP-9 expressed in ectopic endometrium group（32 645±9 967） was higher than that in eutopic endometrium and control groups（P〈0.05）.There was positive correlation between expression of OPN and MMP-9.Conclusion It is high that expression of OPN and MMP-9 in eutopic and ectopic endometrium in rats;There is positive correlation between expression of OPN and MMP-9.They may exist in the regulation of cell information between cascade regulations.